Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin αvβ3-selective cyclic RGD peptides
Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin αvβ3-selective cyclic RGD peptides
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DOI：
10.1002/jbm.a.34303
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Kessler, Horst
中科院分区：
文献类型：
--
作者：
Mas-Moruno, Carlos;Dorfner, Petra M.;Kessler, Horst
众所周知，用模拟细胞外基质蛋白的整合素结合基序的细胞粘附肽对表面进行功能化是改善植入材料的骨整合的可行方法。此外，材料的表面性质的改性（例如，粗糙度）强烈影响细胞行为。然而，这两种方法很少一起研究。本研究提出了这样的假设，即肽功能化和表面粗糙度的组合将对成骨细胞的粘附过程产生增强作用。为了检验这一假设，制备了一系列α v β 3-选择性环状RGD肽，并将其固定在修整（S-a = 0.74 μ m，光滑）和喷砂（S-a = 3.24 μ m，粗糙）的Ti6 Al 4V盘上。这些表面修饰的效果进行了评价方面的整合素α v β 3介导的粘附能力，细胞形态学，和原代人成骨细胞的蔓延。孵育3小时后，成骨细胞粘附更强烈的喷砂比修剪非涂层Ti6 Al 4V表面。它们的附着效率在RGD肽的存在下进一步增强。然而，与喷砂表面相比，肽功能化对修整表面上的成骨细胞附着具有相对较强的影响。培养3小时后的细胞形态完全改变了表面形貌。孵育1 h后，RGD涂层对成骨细胞在修剪和喷砂材料上的扩散至关重要，但3 h后其影响几乎可以忽略不计。这项研究的结果提供的证据表明，联盟的RGD涂层和表面形貌的Ti6 Al 4V积极影响成骨细胞的粘附和扩散，特别是在非常早期的粘附时间。(C)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：8797，2013.
It is well known that functionalization of surfaces with cell adhesive peptides mimicking the integrin binding motif of extracellular matrix proteins is a feasible approach to improve osseointegration of implant materials. Also, modification of the surface properties of the material (e.g., roughness) strongly influences cell behavior. However, these two approaches are rarely studied together. This study addressed the hypothesis that the combination of peptide functionalization and surface roughness will have an enhancing effect on the adhesion process of osteoblasts. To test this hypothesis, a series of alpha v beta 3-selective cyclic RGD peptides were prepared and immobilized on trimmed (S-a = 0.74 mu m, smooth) and sandblasted (S-a = 3.24 mu m, rough) Ti6Al4V disks. Effects of these surface modifications were evaluated with respect to integrin alpha v beta 3-mediated adhesive capacity, cell morphology, and spreading of primary human osteoblasts. After 3 h of incubation, osteoblasts adhered more strongly on sandblasted than on trimmed noncoated Ti6Al4V surfaces. Their attachment efficiency was further enhanced in the presence of RGD peptides. However, peptide functionalization had a relatively stronger impact on osteoblast attachment on trimmed surfaces compared with sandblasted surfaces. Cell morphology after 3 h of culture was exclusively altered by surface topography. RGD coating was critical for osteoblast spreading on both trimmed and sandblasted materials after 1 h of incubation but it showed almost negligible effects after 3 h. The results of this study provide evidence that the alliance of RGD coating and surface topography on Ti6Al4V positively influences osteoblast adhesion and spreading, especially at very early adhesion times. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:8797, 2013.