Bleach interference in forensic luminol tests on porous surfaces: More about the drying time effect
Bleach interference in forensic luminol tests on porous surfaces: More about the drying time effect
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DOI：
10.1016/j.talanta.2008.09.008
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发表时间：
2009-02-15
期刊：
影响因子：
6.1
通讯作者：
Verdu, Fernando
中科院分区：
文献类型：
--
作者：
Castello, Ana;Frances, Francesc;Verdu, Fernando
由于犯罪分子尽量避免在犯罪现场留下线索，血迹往往被冲走，但幸运的是，对于调查人员来说，它们很难完全消除。多孔表面很容易保留血迹，有时肉眼看不到。用于检测所有类型表面上的潜在血迹的试剂选择是鲁米诺，但其主要缺点是对血液样品中的氧化污染物高度敏感。如果使用家用漂白水清洗血迹，推定测试无效。然而，已知次氯酸盐是不稳定的，并且随着时间的推移而恶化，并且该特征可能有助于防止家庭漂白引起的干扰。以前的研究已经评估了干燥时间对无孔表面的影响，但还没有关于多孔表面的这种影响的报道，因此，本文报道了次氯酸盐对用于这种类型表面的鲁米诺试剂的干扰，评估了干燥时间对家用漂白剂-鲁米诺反应的影响，结果表明，该干燥方法可以很好地克服家用漂白剂对鲁米诺的干扰反应测试如果调查允许适当的等待时间。(C)2008 Elsevier B. V.保留所有权利。
As criminals try to avoid leaving Clues at the scene of a crime, bloodstains are often washed away, but fortunately for investigators, they are difficult to eliminate completely. Porous Surfaces easily retain blood traces, which are sometimes invisible to the naked eye. The reagent of choice for detecting latent blood traces on all types of surfaces is luminol, but its main disadvantage is a high degree of sensitivity to oxidising contaminants in the blood sample. If household bleach is used to clean bloodstains, presumptive tests are invalidated. Hypochlorites, however, are known to be unstable and deteriorate over time, and this feature could be of help in preventing household bleach-induced interference. Previous studies have evaluated the effect of the drying time on nonporous surfaces, but nothing has as yet been published about this effect on porous surfaces.Consequently, this paper reports on hypochlorite interference with luminol reagents used on this type of surface, evaluating the effects of drying time on the household bleach-luminol reaction, and ascertaining whether the drying procedure could be applied to prevent household bleach interference on bloodstained porous surfaces.The results indicate that the drying method may very well overcome household bleach interference in luminol reaction tests. if the investigation allows for an appropriate waiting time. (C) 2008 Elsevier B.V. All rights reserved.