Prenatal testosterone and dihydrotestosterone exposure disrupts ovine testicular development.
Prenatal testosterone and dihydrotestosterone exposure disrupts ovine testicular development.
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DOI：
10.1530/rep-10-0210
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发表时间：
2011-07
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影响因子：
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通讯作者：
Lee TM
中科院分区：
文献类型：
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作者：
Bormann CL;Smith GD;Padmanabhan V;Lee TM
雄激素在子宫内生命的前三个月期间发挥重要作用，与生殖道分化一致，在男性化和维持次要男性特征期间，以及在精子发生的启动期间。关于胎儿发育过程中不适当地暴露于过量雄激素对男性性成熟和生殖的影响知之甚少。本研究的目的是确定在绵羊性分化期间产前5α-二氢睾酮（DHT）和睾酮（T）处理对青春期后睾丸形成的影响，以及随后通过附睾精子特征评估的生育潜力。与年龄匹配的对照组（C）和产前DHT处理的公羊相比，产前用T处理的公羊表现出睾丸重量增加（P<0.05），与DHT处理的公羊相比，总T和游离T浓度升高（分别为P=0.07和P<0.05）。与对照组相比，产前经DHT处理的公羊附睾中进行性活动精子的百分比显著降低（P<0.05），而经T处理的公羊则无此效应。T处理的公羊有更多的生殖细胞层比DHT处理的公羊，但与对照组相当。与产前DHT组相比，产前T处理组公羊的曲细精管直径和管腔直径显著增大（P<0.05）。与对照组相比，DHT组和T组产前肾小管管腔闭塞率显著增高（P<0.05）。这项研究的结果表明，暴露于过量的T/DHT在男性胎儿性分化有不同的影响青春期后睾丸的大小，曲细精管的大小和功能，精子活力，和T浓度。
Androgens play important roles during the first trimester of intrauterine life, coinciding with genital tract differentiation, during virilization and maintenance of secondary male characteristics and during initiation of spermatogenesis. Little is known about the impact of inappropriate exposure to excess androgens during fetal development on male sexual maturation and reproduction. The objectives of this study were to determine the effects of prenatal 5α-dihydrotestosterone (DHT) and testosterone (T) treatment during ovine sexual differentiation on post-pubertal testicular formation and subsequent potential for fertility as assessed by epididymal sperm characteristics. Rams prenatally treated with T exhibited increased testicular weight relative to age-matched controls (C) and prenatal DHT-treated rams (P<0.05), as well as elevated total and free T concentrations compared to DHT-treated rams (P=0.07 and P<0.05, respectively). The percentage of progressively motile sperm from the epididymis was significantly reduced in prenatal DHT-treated but not T-treated rams compared to C rams (P<0.05). The T-treated rams had a greater number of germ cell layers than DHT-treated rams, but comparable to the controls. Prenatal T-treated rams had significantly larger seminiferous tubule diameter, and lumen diameter compared to prenatal DHT-treated (P<0.05). Significantly more prenatal DHT- and T-treated rams (P<0.05) had occluded tubule lumen than C rams. Findings from this study demonstrate that exposure to excess T/DHT during male fetal sexual differentiation have differential effects on post-pubertal testicular size, seminiferous tubule size and function, sperm motility, and T concentrations.