Pathogenesis and Immune Response in Resistant and Susceptible Cultivars of Grapevine (Vitis spp.) Against Elsinoë ampelina Infection
Pathogenesis and Immune Response in Resistant and Susceptible Cultivars of Grapevine (Vitis spp.) Against Elsinoë ampelina Infection
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葡萄（Vitis spp.）抗 Elsinoñ ampelina 感染的抗性和敏感品种的发病机制和免疫反应
DOI：
10.1094/phyto-03-20-0079-r
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Xiping Wang
中科院分区：
文献类型：
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作者：
Rui Han;Wuchen Yin;Bilal Ahmad;Peijia Gao;Zhi Li;Xiping Wang
葡萄炭疽病是葡萄炭疽病的主要病原，大多数葡萄品种对该真菌都很敏感。然而，一些中国野生葡萄品种是抗病的。因此，比较感病和抗病葡萄品种的致病机理和免疫反应，有助于探索葡萄对该真菌的详细分子和生化抗性机制。本研究采用超微结构和组织病理学方法，研究了中国野生葡萄抗病品种‘尚-24’和感病品种‘糖味’和‘汤普森无核’对鹅掌叶葡萄的抗性反应。接种鹅膏菌72h后，感病品种‘糖味’和‘汤普森无核’叶片上可见明显的褐色坏死斑。侵染的特点是菌丝对寄主细胞的快速定植和病原菌在细胞间隙的大量传播，最终导致寄主细胞崩溃、角质层溶解和菌丝广泛生长。抗病无性系‘尚-24’的分生孢子裂解，出现大量电子致密物质，菌丝生长受到抑制，寄主细胞保持完整。此外，6个与抗病相关的基因在感病和抗病品种中也有差异表达。感染鹅膏菌后，这些疾病相关基因的表达显著上调。本研究阐明了鹅膏菌在抗病和感病葡萄叶片中侵染和定殖的差异。
Elsinoë ampelina is the main cause of grape anthracnose, and the majority of grapevine cultivars are susceptible to this fungus. Some Chinese wild grape cultivars are resistant, however. It is therefore apt to compare the pathogenesis and immune responses in susceptible and resistant cultivars of grapevine to explore the detailed molecular and biochemical mechanisms of resistance to this fungus. In this study, ultrastructural and histopathological observations were used to demonstrate the resistance responses to E. ampelina in the resistant Chinese wild cultivar Vitis quinquangularis clone ‘Shang-24’ and the susceptible cultivars V. davidii ‘Tangwei’ and V. vinifera ‘Thompson Seedless’. Seventy-two hours postinoculation (hpi) with E. ampelina, brown necrotic spots were clearly visible on the leaves of the susceptible ‘Tangwei’ and ‘Thompson Seedless’. The infection was characterized by rapid colonization of the host cells by hyphae and massive spread of the pathogen in the intercellular spaces, ultimately leading to host cell collapse, cuticle dissolution, and extensive hyphal growth. In the resistant clone ‘Shang-24’, the conidia were lysed, a large quantity of electronically dense matter appeared, the hyphal growth was suppressed, and the host cells remained intact. In addition, six genes associated with disease resistance were differentially expressed in the susceptible and resistant cultivars. These disease-related genes were significantly up-regulated following infection with E. ampelina. This study illustrates the differences in infection and colonization of E. ampelina in resistant and susceptible grape leaves.