On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow
On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow
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DOI：
10.1038/micronano.2015.1
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发表时间：
2015-01-01
影响因子：
7.9
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Hayakawa, Takeshi;Sakuma, Shinya;Arai, Fumihito
我们提出了一种基于振动诱导流的片上三维细胞旋转方法。当圆振动作用于具有微柱图案的微芯片时，微柱周围会产生高度局部化的旋转流。这种流动的方向和速度可以通过改变施加振动的方向和振幅来控制。此外，这种流动可以在开放芯片结构上诱导。在本研究中，我们采用了三根微柱呈三角形排列的微芯片和xyz压电驱动器来施加圆形振动。在微柱的中心，源自单个微柱的振动诱导流动的干涉引起了旋转流动。因此，放置在这个中心的生物细胞在水流的影响下旋转。在xy， xz或yz平面的三平面圆形振动下，细胞可以在显微镜的焦平面和垂直平面上旋转。利用这种三维细胞旋转方法，我们测量了小鼠卵母细胞在焦平面和垂直平面上的旋转速度分别为63.7 +/- 4.0度s（-1）和3.5 +/- 2.1度s（-1）。此外，我们展示了小鼠卵母细胞的运输和旋转，并将其细胞核重新定位到显微镜下可观察的位置。
We propose a novel on-chip 3D cell rotation method based on a vibration-induced flow. When circular vibration is applied to a microchip with micropillar patterns, a highly localized whirling flow is induced around the micropillars. The direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied vibration. Furthermore, this flow can be induced on an open chip structure. In this study, we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular vibration. At the centre of the micropillars, the interference of the vibration- induced flows originating from the individual micropillars induces rotational flow. Consequently, a biological cell placed at this centre rotates under the influence of the flow. Under three-plane circular vibrations in the xy, xz or yz plane, the cell can rotate in both the focal and vertical planes of the microscope. Applying this 3D cell rotation method, we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7 +/- 4.0 degrees s(-1) and 3.5 +/- 2.1 degrees s(-1), respectively. Furthermore, we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.