A YEAST REPLICATION ORIGIN CONSISTS OF MULTIPLE COPIES OF A SMALL CONSERVED SEQUENCE
A YEAST REPLICATION ORIGIN CONSISTS OF MULTIPLE COPIES OF A SMALL CONSERVED SEQUENCE
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DOI：
10.1016/0092-8674(88)90164-x
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发表时间：
1988-05-06
期刊：
影响因子：
64.5
通讯作者：
NEWLON, CS
中科院分区：
文献类型：
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作者：
PALZKILL, TG;NEWLON, CS
酿酒酵母的自主复制序列（ars）在质粒和染色体上起着复制起点的作用。ARS功能需要ARS核心共识（5”-[a /T]TTTAT[a /G]TTT[a /T]-3”）的副本和共识的T-rich链的附加序列3”。我们对来自染色体III的一个ARS，即C2G1 ARS的分析表明，ARS的功能取决于是否存在与核心共识的精确匹配，以及是否存在额外的3”侧翼区域的近匹配。我们已经证明，通过从含有共识序列副本的寡核苷酸构建合成ARS元件，ARS功能可以通过与核心共识的多个匹配来介导。我们发现，核心共识的两个副本足以使ARS活动，并且可以在特定方向上由多个核心共识元素构建一个与天然染色体ARS一样高效的人工ARS。
Autonomously replicating sequences (ARSs) of the yeast S. cerevisiae function as replication origins on plasmids and probably also on chromosomes. ARS function requires a copy of the ARS core consensus (5''-[A/T]TTTAT[A/G]TTT[A/T]-3'') and additional sequences 3'' to the T-rich strand of the consensus. Our analysis of an ARS from chromosome III, the C2G1 ARS, suggests that ARS function depends on the presence of an exact match to the core consensus and the presence of additional near matches in the 3''flanking region. We have demonstrated that ARS function can be mediated by multiple matches to the core consensus by constructing synthetic ARS elements from oligonucleotides containing copies of the consensus sequence. We find that two copies of the core consensus are sufficient for ARS activity and that an artificial ARS as efficient as a natural chromosomal ARS can be constructed from multiple core consensus elements in a specific orientation.