Viable Tumor Tissue Detection in Murine Metastatic Breast Cancer by Whole-Body MRI and Multispectral Analysis
Viable Tumor Tissue Detection in Murine Metastatic Breast Cancer by Whole-Body MRI and Multispectral Analysis
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DOI：
10.1002/mrm.22109
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发表时间：
2009-12-01
影响因子：
3.3
通讯作者：
Carano, Richard A. D.
中科院分区：
文献类型：
--
作者：
Barck, Kai H.;Willis, Brandon;Carano, Richard A. D.
全身MRI结合半自动分层多光谱图像分析技术进行了评价，作为检测转移性乳腺癌（4 T1细胞系）小鼠模型中活肿瘤组织的方法。在这项研究中获得了全身表观扩散系数，T-2和质子密度图。可行的肿瘤组织分割包括三个阶段的k均值聚类的参数图，形态学操作，应用程序的大小阈值，和读者的歧视分割的对象。通过组织学评价对分割结果进行了验证，并在三个尺寸阈值（15，100和500体素）下评估了该技术的检测准确性。对于500体素大小阈值，准确率为88.9%，受试者工作特征曲线下面积为0.84。在原发性肿瘤内发现的分段存活肿瘤组织区域主要位于肿瘤的外周，与组织学结果一致。所提出的技术被发现能够检测转移和分割的肿瘤坏死区域内的肿瘤在这个模型中发现的活的肿瘤。它为临床前和潜在的临床应用提供了一种非侵入性、全身、有活力的肿瘤组织检测方法，如肿瘤筛查和评估治疗效果。Magn Reson Med 62：1423-1430，2009。(C)2009 Wiley-Liss，Inc.
Whole-body MRI combined with a semiautomated hierarchical multispectral image analysis technique was evaluated as a method for detecting viable tumor tissue in a murine model of metastatic breast cancer (4T1 cell line). Whole-body apparent diffusion coefficient, T-2, and proton density maps were acquired in this study. The viable tumor tissue segmentation included three-stage k-means clustering of the parametric maps, morphologic operations, application of a size threshold, and reader discrimination of the segmented objects. The segmentation results were validated by histologic evaluation, and the detection accuracy of the technique was evaluated at three size thresholds (15, 100, and 500 voxels). The accuracy was 88.9% for a 500-voxel size threshold, and the area under receiver operating characteristic curve was 0.84. The regions of segmented viable tumor tissue within the primary tumors were found mostly on the periphery of the tumors in agreement with the histologic findings. The presented technique was found capable of detecting metastases and segmenting the viable tumor from necrotic regions within tumors found in this model. It offers a noninvasive, whole-body, viable tumor tissue detection method for preclinical and potentially clinical applications such as tumor screening and evaluating therapeutic efficacy. Magn Reson Med 62:1423-1430, 2009. (C) 2009 Wiley-Liss, Inc.