The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles
The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles
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DOI：
10.1262/jrd.2019-112
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发表时间：
2019-12-01
影响因子：
1.8
通讯作者：
Funahashi, Hiroaki
中科院分区：
文献类型：
--
作者：
Kohata-Ono, Chiyuki;Wakai, Takuya;Funahashi, Hiroaki
本研究旨在探讨自噬诱导剂雷帕霉素（rapamycin）和自噬抑制剂3-甲基腺嘌呤（3-methyladenine，3-MA）对猪中卵泡（MF，直径3-6 mm）和小卵泡（SF，直径1-2 mm）卵母细胞体外成熟（IVM）过程中减数分裂和发育能力的影响。1 nM雷帕霉素的存在显著提高了MF衍生的卵母细胞的成熟率（P < 0.05），而10 nM雷帕霉素的存在没有显著提高MF衍生的卵母细胞的成熟率（P < 0.05）。然而，SF衍生的卵母细胞的成熟率不受雷帕霉素在两个浓度（1 nM和10 nM）。与未添加3-MA的对照组相比，添加0.2mM而非2 mM 3-MA的MF衍生卵母细胞的成熟率显著降低（P < 0.05）。相反，在SF衍生的卵母细胞中，0.2和2 mM浓度的3-MA不影响成熟率。1 nM雷帕霉素的存在显著增加MF衍生的成熟卵母细胞孤雌激活后的囊胚形成率（P < 0.05）。然而，SF衍生的成熟卵母细胞的囊胚形成率不受雷帕霉素的存在。3-MA的存在显着降低MF衍生的成熟卵母细胞的囊胚形成率，但没有改变SF衍生的卵母细胞。总之，我们的研究结果表明，自噬诱导剂和抑制剂对MF和SF衍生的猪卵母细胞的减数分裂和发育能力的活性存在差异。
This study aimed to examine the effect of rapamycin (autophagy inducer) and 3-methyladenine (3-MA, autophagy inhibitor) on the meiotic and developmental competencies of porcine oocytes derived from medium follicles (MF, 3-6 mm in diameter) and small follicles (SF, 1-2 mm in diameter) during in vitro maturation (IVM) process. The presence of 1 nM but not 10 nM rapamycin significantly increased the maturation rate of MF-derived oocytes (P < 0.05). However, the maturation rate of SF-derived oocytes was not affected by rapamycin at both concentrations (1 nM and 10 nM). The maturation rate of MF-derived oocytes decreased significantly (P < 0.05) in the presence of 0.2 mM but not 2 mM 3-MA than non-supplemented control. In contrast, in SF-derived oocytes, 3-MA at both 0.2 and 2 mM concentrations did not affect the maturation rates. The presence of 1 nM rapamycin significantly increased the blastocyst formation rate of MF-derived mature oocytes following parthenogenetic activation (P < 0.05). However, the blastocyst formation rate of SF-derived mature oocytes was not affected by the presence of rapamycin. The presence of 3-MA significantly reduced the blastocyst formation rate of MF-derived mature oocytes but did not change that of SF-derived oocytes. In conclusion, our study results show differences in activity of the autophagy inducer and inhibitor on the meiotic and developmental competencies of MF- and SF-derived porcine oocytes.