Additional member of the protein-tyrosine kinase family: the src- and lck-related protooncogene c-tkl.
Additional member of the protein-tyrosine kinase family: the src- and lck-related protooncogene c-tkl.
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蛋白酪氨酸激酶家族的其他成员：src 和 lck 相关的原癌基因 c-tkl。
DOI：
10.1073/pnas.84.24.8778
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发表时间：
1987
影响因子：
11.1
通讯作者：
Rübsamen-Waigmann,H
中科院分区：
文献类型：
--
作者：
Strebhardt,K;Mullins,JI;Bruck,C;Rübsamen-Waigmann,H
我们报告的分离和核苷酸序列的3.7-脱氢酶（kb）的cDNA克隆从鸡脾脏对应于一个以前未描述的成员src家族的原癌基因。它编码具有与蛋白酪氨酸激酶的src家族相关的C-末端结构域的蛋白（EC 2.7.1.112），并且在这些蛋白中，与从鼠白血病病毒诱导的胸腺瘤细胞系分离的lck基因具有最显著的同源性。因此，该基因被称为c-tkl，是与lck相关的细胞酪氨酸激酶。基因组DNA的分析揭示c-tkl是不同于c-src和c-lck的染色体位点。此外，c-tkl mRNA的大小及其表达模式表明它不是lck的鸡同源物，而是一个不同的基因。在培养的鸡胚成纤维细胞和鸡脾脏和脑中观察到c-tkl基因的3.8 kb转录本，与c-src mRNA的大小相同。与此相反，当用v-src的5'末端作为探针时，在所采用的杂交条件下，用来自脾的mRNA检测到明确的c-src mRNA信号是不可能的，并且从cDNA文库获得的11个克隆中没有一个对应于c-src转录本。因此，以前的研究c-src mRNA在脾脏中的表达可能实际上检测到c-tkl转录本。
We report the isolation and nucleotide sequence of a 3.7-kilobase (kb) cDNA clone from chicken spleen corresponding to a previously undescribed member of the src family of protooncogenes. It encodes a protein with a C-terminal domain related to the src family of protein-tyrosine kinases (EC 2.7.1.112) and, among these, has most significant homology to the lck gene isolated from a murine leukemia virus-induced thymoma cell line. The gene is therefore referred to as c-tkl for cellular tyrosine kinase related to lck. Analysis of genomic DNA reveals that c-tkl is a chromosomal locus distinct from c-src and c-lck. Furthermore, the size of c-tkl mRNA as well as its pattern of expression indicates that it is not the chicken homologue of lck but a different gene. A 3.8-kb transcript of the c-tkl gene, identical to the size determined for c-src mRNA, was observed in cultured chicken embryo fibroblasts and in chicken spleen and brain. In contrast, detection of a definite c-src mRNA signal with mRNA from spleen was not possible under the hybridization conditions employed when the 5' end of v-src was used as the probe, and none of the 11 clones obtained from the cDNA library corresponded to a c-src transcript. Thus previous studies of c-src mRNA expression in spleen may have actually detected c-tkl transcripts.