Effects of surface treatments on trapping with DC insulator-based dielectrophoresis
Effects of surface treatments on trapping with DC insulator-based dielectrophoresis
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DOI：
10.1039/c9an01186b
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发表时间：
2019-12-21
期刊：
影响因子：
4.2
通讯作者：
Gile, Gillian H.
中科院分区：
文献类型：
--
作者：
Crowther, Claire, V;Sanderlin, Viola;Gile, Gillian H.
用电动方法测量细胞的生物物理特性的一个中心挑战是将这些生物物理特性分配给特定的生物学特性。细胞电动行为的变化可能来自突变、基因表达水平的改变、翻译后的修饰或环境影响。在这里，我们评估化学表面修饰的细菌细胞的电动行为，以深入了解表面化学变化特别影响的生物物理性质。以大肠杆菌为支架，通过胺偶联反应将甘氨酸、精胺、牛血清白蛋白(蛋白质)或7-氨基-4-甲基-3-香豆素乙酸(荧光染料)共价连接到细胞表面的游离羧酸基团上。这些群体与未标记的对照细胞一起，接受电动和介电泳法测量，以量化改变后生物物理性质的任何变化。讨论了与所使用的特定反应物相关的每种电动动力的性质。我们的结论是，细胞表面相对温和和表面的变化可以导致其生物物理性质的可测量变化。
A central challenge in measuring the biophysical properties of cells with electrokinetic approaches is the assignment of these biophysical properties to specific biological characteristics. Changes in the electrokinetic behavior of cells may come from mutations, altered gene expression levels, post-translation modifications, or environmental effects. Here we assess the electrokinetic behavior of chemically surface-modified bacterial cells in order to gain insight into the biophysical properties that are specifically affected by changes in surface chemistry. Using E. coli as a scaffold, an amine coupling reaction was used to covalently attach glycine, spermine, bovine serum albumin (protein), or 7-amino-4-methyl-3-coumarinylacetic acid (fluorescent dye) to the free carboxylic acid groups on the surface of the cells. These populations, along with unlabeled control cells, were subject to electrokinetic and dielectrophoretic measurements to quantify any changes in the biophysical properties upon alteration. The properties associated with each electrokinetic force are discussed relative to the specific reactant used. We conclude that relatively modest and superficial changes to cell surfaces can cause measurable changes in their biophysical properties.