INTERGENERIC COAGGREGATION OF ORAL TREPONEMA SPP WITH FUSOBACTERIUM SPP AND INTRAGENERIC COAGGREGATION AMONG FUSOBACTERIUM SPP
INTERGENERIC COAGGREGATION OF ORAL TREPONEMA SPP WITH FUSOBACTERIUM SPP AND INTRAGENERIC COAGGREGATION AMONG FUSOBACTERIUM SPP
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DOI：
10.1128/iai.63.12.4584-4588.1995
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发表时间：
1995-12-01
影响因子：
3.1
通讯作者：
GREENBERG, EP
中科院分区：
文献类型：
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作者：
KOLENBRANDER, PE;PARRISH, KD;GREENBERG, EP
共有22株密螺旋体与7株口腔梭杆菌、2株非口腔梭杆菌和45株其他口腔细菌共聚集，包括放线菌、放线菌、嗜碳细胞菌、真细菌、卟啉单胞菌、普氏菌、硒单胞菌、链球菌和细孔菌。所有密螺旋体、8株密螺旋体、8株socranskii密螺旋体、4株口腔溶果胶密螺旋体、1株果胶ovorum密螺旋体和1株文森螺旋体均与至少1株作为合作伙伴的梭杆菌聚集，其中包括1株牙周梭杆菌。在观察到的100多个聚集中，梭杆菌伴侣被热灭活（85℃30 min），而密螺旋体不受热处理的影响。此外，梭杆菌通常被蛋白酶K处理而灭活，而密螺旋体不受影响。只有乳糖和d -半乳糖胺能抑制T. denticola的聚集，其余23种糖和l -精氨酸均不能抑制T. denticola的聚集。在核仁F.和牙周F.的亚种之间观察到基因内聚集，并且没有被任何测试的糖或l -精氨酸抑制，在密螺旋体之间没有观察到基因内聚集。这些数据表明，人类口腔密螺旋体显示出口腔梭杆菌作为共聚集伙伴的特异性，这种细胞间接触可能促进有效的代谢交流，并在牙周病逐渐加重的阶段增强每个细胞的增殖。
A total of 22 strains of Treponema spp, including members of all four named human oral species were tested for coaggregation with 7 strains of oral fusobacteria, 2 strains of nonoral fusobacteria, and 45 strains of other oral bacteria, which included actinobacilli, actinomyces, capnocytophagae, eubacteria, porphyromonads, prevotellae, selenomonads, streptococci, and veillonellae. None of the treponemes coaggregated with any of the latter 45 oral strains or with the two nonoral fusobacteria, All treponemes, eight Treponema denticola strains, eight T. socranskii strains, four oral pectinolytic treponemes, one T. pectinovorum strain, and one T. vincentii strain coaggregated with at least one strain of the fusobacteria tested as partners, The partners consisted of one strain of Fusobacterium periodonticum, five F. nucleatum strains including all four subspecies of F. nucleatum, and a strain of F. simiae obtained from the dental plaque of a monkey, In the more than 100 coaggregations observed, the fusobacterial partner was heat inactivated (85 degrees C for 30 min), while the treponemes were unaffected by the heat treatment, Furthermore, the fusobacteria were usually inactivated by proteinase K treatment, and the treponemes were not affected, Only the T. denticola coaggregations were inhibited by lactose and D-galactosamine, None were inhibited by any of 23 other different sugars or L-arginine. Intrageneric coaggregations were seen among the subspecies of F. nucleatum and with F. periodonticum, and none were inhibited by any of the sugars tested or by L-arginine, No intrageneric coaggregations were observed among the treponemes. These data indicate that the human oral treponemes show a specificity for oral fusobacteria as coaggregation partners, Such cell-to-cell contact may facilitate efficient metabolic communication and enhance the proliferation of each cell in the progressively more severe stages of periodontal disease,