An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers.
An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers.
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DOI：
10.1016/j.talanta.2012.02.046
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发表时间：
2012-05
期刊：
影响因子：
6.1
通讯作者：
Zhexiang Zou;Jun Wang;Hua Wang;Yaoyao Li;Yuehe Lin
中科院分区：
文献类型：
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作者：
Zhexiang Zou;Jun Wang;Hua Wang;Yaoyao Li;Yuehe Lin
提出了一种新型的电化学生物传感装置，该装置将免疫层析试纸条和连接到便携式电化学分析仪的丝网印刷电极（SPE）集成在一起，用于快速、灵敏、定量地检测人类血液样本中与疾病相关的生物标志物。该传感器的原理是基于生物标志物与测试条上的一对抗体之间的夹心免疫反应，然后是高灵敏度的方波伏安法（SWV）检测。用辣根过氧化物酶（HRP）作为电化学读出的信号报告蛋白。本研究采用乙型肝炎表面抗原（HBsAg）作为模型蛋白生物标志物来验证该传感器的分析性能。对控制传感器性能的一些关键参数进行了详细的研究。在最佳条件下，该传感器能检测出最低0.3ngmL−1（S/N=3）的HBsAg，线性浓度范围为1 ~ 500ngmL−1。该传感器进一步用于检测人血浆中HBsAg，平均回收率为91.3%。与之相比，还进行了比色免疫层析试验条法（ITSA）。结果表明，电化学传感器中的SWV检测对HBsAg的定量检测比比色检测灵敏得多，这表明该传感器是一个有前景的平台，可以在大量人群中快速、灵敏地进行疾病相关生物标志物的即时检测/筛选。
A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3ngmL−1(S/N=3) HBsAg with a wide linear concentration range from 1 to 500ngmL−1. The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population.