Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells
Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells
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DOI：
10.1016/s0736-0266(02)00261-9
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发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Puzas, JE
中科院分区：
文献类型：
--
作者：
Boyan, BD;Schwartz, Z;Puzas, JE
种植体表面形态调控成骨细胞表型表达。成骨细胞对非生物表面的敏感性表明，天然骨表面特征也可能影响成骨细胞的反应。为了验证这一点，MG63成骨样细胞在用大鼠骨髓破骨细胞预处理0、10或20天的牛皮质骨薄片上生长7天。将MG63细胞对破骨细胞处理表面的反应与光滑和粗糙微形貌的钛表面的反应进行比较。在融合培养中测量细胞数量、分化（碱性磷酸酶活性和骨钙素水平）和局部因子（PGE(2)和tgf - β 1）。与塑料培养相比，三种骨片上的细胞数量均减少；这种效应随表面吸收的增加呈剂量依赖性。与塑料相比，三种表面的碱性磷酸酶比活性均有所增加（P < 0.05），但这种增加与吸收时间无关，表明该参数对表面（牛骨与塑料）敏感，但对破骨细胞吸收不敏感。骨表面吸收面积与骨钙素、tgf - β 1、PGE含量呈正相关（R-2分别为0.8025、0.8689、0.8896）。经过20天的破骨细胞预处理，骨钙素比塑料增加了20倍，比未经处理的骨薄片增加了7倍。tgf - β 1和PGE2也有类似的增加。因此，破骨细胞预处理引起的表面变化对成骨细胞表型表达有很强的影响，表明微地形可能起作用。(C) 2003骨科研究学会。Elsevier Science Ltd出版，版权所有。
Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P less than or equal to 0.05) on all three surfaces compared with plastic, but this increase was not dependent on resorption time, indicating this parameter was sensitive to the surface (bovine bone vs. plastic) but not to osteoclast-resorption. There was a direct correlation between the area of the bone surface resorbed and the amount of osteocalcin, TGF-beta1 and PGE(2) (R-2 = 0.8025, 0.8689, 0.8896, respectively). With 20 days of osteoclast pretreatment, there was a 20-fold increase in osteocalcin over plastic and a 7-fold increase over cultures on untreated bone wafers. Similar increases were found for TGF-beta1 and PGE2. Thus, surface changes resulting from osteoclast pretreatment have a strong effect on osteoblast phenotypic expression, and suggest that microtopography may play a role. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd, All rights reserved.