Microtubule-dependent membrane dynamics in Ustilago maydis: Trafficking and function of Rab5a-positive endosomes.
Microtubule-dependent membrane dynamics in Ustilago maydis: Trafficking and function of Rab5a-positive endosomes.
复制标题
DOI：
10.4161/cib.21219
复制
发表时间：
2012-09-01
影响因子：
--
通讯作者：
Feldbrügge M
中科院分区：
文献类型：
--
作者：
Göhre V;Vollmeister E;Bölker M;Feldbrügge M
膜结构沿着细胞骨架的长距离运输对于真核生物的分泌和内吞作用至关重要。分子马达沿着沿着极化微管运输分泌和内吞囊泡。在这里，我们回顾了模式微生物玉米黑粉菌中以G蛋白Rab 5a为标志的大囊泡的运输机制和生物学功能。这些Rab 5a阳性内体通过Unc 104/KIF 1A相关的运动因子Kin 3和动力蛋白Dyn 1/2介导沿微管双向沿着穿梭。Rab 5a阳性内体在U.玉米粉在单倍体出芽细胞中，它们参与胞质分裂和信息素信号传导。在丝状生长期间，内体用于mRNA的长距离运输，这是维持极性的先决条件，最有可能通过在丝状体的顶端和远端的特定蛋白质的局部翻译。mRNA的内体共转运构成了这些膜隔室的新功能，支持内体作为多用途平台发挥功能的观点。
Long-distance trafficking of membranous structures along the cytoskeleton is crucial for secretion and endocytosis in eukaryotes. Molecular motors are transporting both secretory and endocytic vesicles along polarized microtubules. Here, we review the transport mechanism and biological function of a distinct subset of large vesicles marked by the G-protein Rab5a in the model microorganism Ustilago maydis. These Rab5a-positive endosomes shuttle bi-directionally along microtubules mediated by the Unc104/KIF1A-related motor Kin3 and dynein Dyn1/2. Rab5a-positive endosomes exhibit diverse functions during the life cycle of U. maydis. In haploid budding cells they are involved in cytokinesis and pheromone signaling. During filamentous growth endosomes are used for long-distance transport of mRNA, a prerequisite to maintain polarity most likely via local translation of specific proteins at both the apical and distal ends of filaments. Endosomal co-transport of mRNA constitutes a novel function of these membrane compartments supporting the view that endosomes function as multipurpose platforms.