Unusual responses of cultured Fundulus heteroclitus melanophores to epinephrine and ions, paradox of K+ versus Na+.
Unusual responses of cultured Fundulus heteroclitus melanophores to epinephrine and ions, paradox of K+ versus Na+.
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培养的眼底异斜黑色素细胞对肾上腺素和离子的异常反应，K 与 Na 的悖论。
DOI：
10.1002/jez.1402360314
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Tchen,TT
中科院分区：
文献类型：
--
作者：
Clark,CR;Taylor,JD;Tchen,TT
成功地培养了异形藻黑素载体，并在培养中保存了长达2个月。与鳍、鳞或裂鳍制剂中的其他硬骨黑素载体相比，培养的黑素载体对肾上腺素和离子溶液都显示出不寻常的反应。首先，它们聚集自己的黑素小体，以响应低至10−12M的肾上腺素浓度。其次，在6天或更长时间的培养中，黑素载体聚集了他们的黑素小体，对钠离子和钾离子溶液都有反应。这与4天前的培养(以及未培养的黑素细胞的报道)形成对比，在培养过程中，黑素小体对钾的反应是聚集的，但在钠离子溶液中分散。
Fundulus heteroclitus melanophores were successfully cultured and maintained in culture for up to 2 months. Compared to other teleost melanophores in the fin, scale, or split fin preparation, the cultured melanophores show unusual responses to both epinephrine and ion solutions. First, they aggregated their melanosomes in response to concentrations of epinephrine as low as 10−12M. Second, the melanophores in a 6‐day, or older, culture aggregated their melanosomes in response to both sodium and potassium ion solutions. This is in contrast to 4‐day‐old cultures (and reports of noncultured melanophores) where melanosomes are aggregated in response to potassium but dispersed in sodium ion solutions.