Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses
Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses
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DOI：
10.1128/jcm.38.9.3492-3494.2000
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发表时间：
2000-09
影响因子：
9.4
通讯作者：
Shinichi Kobayashi;K. Sakae;Yasumoto Suzuki;K. Shinozaki;M. Okada;H. Ishiko;K. Kamata;Kenji Suzuki;K. Natori;T. Miyamura;N. Takeda
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文献类型：
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作者：
Shinichi Kobayashi;K. Sakae;Yasumoto Suzuki;K. Shinozaki;M. Okada;H. Ishiko;K. Kamata;Kenji Suzuki;K. Natori;T. Miyamura;N. Takeda
用杆状病毒表达系统表达基因组I诺瓦克样病毒（NLV）日本株濑户病毒（SeV）的病毒衣壳蛋白。基于重组SeV的超免疫抗血清的抗原检测酶联免疫吸附试验对粪便中的同源SeV样株而不是异源株具有高度特异性，从而使我们能够对NLV进行类型特异性检测。
ABSTRACT The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.