Detection of Chlorogenic Acid in Honeysuckle Using Infrared-Assisted Extraction Followed by Capillary Electrophoresis with UV Detector
Detection of Chlorogenic Acid in Honeysuckle Using Infrared-Assisted Extraction Followed by Capillary Electrophoresis with UV Detector
复制标题
DOI：
10.1093/chromsci/bmr003
复制
发表时间：
2012-01-01
影响因子：
1.3
通讯作者：
Zhang, Xiangmin
中科院分区：
文献类型：
--
作者：
Tang, Zhuxing;Zang, Shuliang;Zhang, Xiangmin
本研究采用红外辅助萃取耦合毛细管电泳（CE）方法测定中药金银花中绿原酸的含量。考察了pH、缓冲液浓度、分离电压、注射时间、红外照射时间、无水乙醇等因素对提取浓度的影响。最佳提取条件为：提取时间30 min；萃取溶剂：80% （v/v）乙醇水溶液；以50 mmol/L硼酸盐缓冲液（pH 8.7）作为运行缓冲液，分离电压为16 kV。样品在16 kV下通电注入8 s。在所研究的浓度范围内呈良好的线性关系（r(2) > 0.9996），且溶液稳定性高。绿原酸加样回收率为95.53% ~ 106.62%，相对标准偏差小于4.1%。采用这种红外辅助提取方法，其提取效率高于传统热回流提取方法。所建立的红外辅助提取方法简单、成本低、效率高，为中药中有效成分的快速测定提供了良好的前景。结果表明，红外辅助萃取-毛细管电泳是一种可靠的中药有效成分定量分析方法。
In this study, a novel infrared-assisted extraction method coupled capillary electrophoresis (CE) is employed to determine chlorogenic acid from a traditional Chinese medicine (TCM), honeysuckle. The effects of pH and the concentration of the running buffer, separation voltage, injection time, IR irradiation time, and anhydrous ethanol in the extraction concentration were investigated. The optimal conditions were as follows: extraction time, 30 min; extraction solvent, 80% (v/v) ethanol in water solution; and 50 mmol/L borate buffer (pH 8.7) was used as the running buffer at a separation voltage of 16 kV. The samples were injected electrokinetically at 16 kV for 8 s. Good linearity (r(2) > 0.9996) was observed over the concentration ranges investigated, and the stability of the solutions was high. Recoveries of the chlorogenic acid were from 95.53% to 106.62%, and the relative standard deviation was below 4.1%. By using this novel IR-assisted extraction method, a higher extraction efficiency than those extracted with conventional heat-reflux extraction was found. The developed IR-assisted extraction method is simple, low-cost, and efficient, offering a great promise for the quick determination of active compounds in TCM. The results indicated that IR-assisted extraction followed by CE is a reliable method for quantitative analysis of active ingredient in TCM.