Analysis of TNF-alpha-mediated cerebral pericyte remodeling.
Analysis of TNF-alpha-mediated cerebral pericyte remodeling.
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TNF-α介导的脑周细胞重塑分析。
DOI：
10.1007/978-1-4939-0669-7_8
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Milner,Richard
中科院分区：
文献类型：
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作者：
Welser-Alves,JenniferV;Boroujerdi,Amin;Tigges,Ulrich;Milner,Richard
TNF-α不仅是炎症和免疫介导事件的中枢调节剂，还影响血管重塑，导致血管结构和功能的改变。除了内皮细胞外，周细胞是另一种血管细胞，对血管的发育、成熟、稳定和重塑起着重要作用。为了研究不同因素对周细胞行为的调控影响，我们最近描述了一种新颖而简单的方法，即分离和培养高纯度、高密度的小鼠脑周细胞。在本章中，我们简要地描述了这种培养系统，以及检查周细胞行为的不同方面的方法，包括细胞粘附、细胞迁移和细胞增殖。这些试验可用于检查TNF-α或任何其他因素对周细胞行为的影响。
As well as being a central regulator of inflammatory and immune-mediated events, TNF-α also influences vascular remodeling, resulting in alterations in the structure and function of blood vessels. In addition to endothelial cells, pericytes are another type of vascular cell that significantly contribute to the development, maturation, stabilization, and remodeling of blood vessels. To investigate the regulatory influence of different factors on pericyte behavior, we recently described a novel yet simple approach of isolating and culturing highly pure, high density cultures of mouse brain pericytes. In this chapter, we briefly describe this culture system, as well as methods for examining different aspects of pericyte behavior, including cell adhesion, cell migration, and cell proliferation. These assays can be used to examine the influence of TNF-α or any other factor on pericyte behavior.