Characterization of nitrogen-fixing Paenibacillus species by polymerase chain reaction-restriction fragment length polymorphism analysis of part of genes encoding 16S rRNA and 23S rRNA and by multilocus enzyme electrophoresis
Characterization of nitrogen-fixing Paenibacillus species by polymerase chain reaction-restriction fragment length polymorphism analysis of part of genes encoding 16S rRNA and 23S rRNA and by multilocus enzyme electrophoresis
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DOI：
10.1016/s0378-1097(03)00300-8
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发表时间：
2003-05-28
影响因子：
2.1
通讯作者：
Seldin, L
中科院分区：
文献类型：
--
作者：
Coelho, MRR;von der Weid, I;Seldin, L
通过对聚合酶链式反应扩增的部分 16S 和 23S rRNA 基因进行限制性片段长度多态性 (RFLP) 分析，对代表 8 个已识别固氮类芽孢杆菌属物种的 42 个菌株和 12 个未识别菌株进行了检查。 （PCR）。通过四种核酸内切酶的 RFLP 分析组合数据获得了 11 种不同的 16S rDNA 基因型，它们与已建立的分类学分类一致。只有一组未分类的菌株（第 1 组）被分配为单独的基因型，表明它们属于一个新物种。使用23S PCR RFLP方法仅检测到六种基因型，表明该方法的辨别力低于16S PCR-RFLP。使用多位点酶电泳 (MLEE) 分析，测试的 48 个菌株可分为 35 个酶变体。测试的七个酶基因座是多态性的，并且在菌株之间获得的不同图谱允许将菌株分为 10 个簇。 PCR-RFLP 方法与 MLEE 测定相结合，为表征和建立属于该固氮类群的分离株的分类位置提供了快速工具，该固氮类群在促进植物生长方面显示出巨大的潜力。 (C) 2003 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Forty-two strains representing the eight recognized nitrogen-fixing Paenibacillus species and 12 non-identified strains were examined by restriction fragment length polymorphism (RFLP) analysis of part of 16S and 23S rRNA genes amplified by polymerase chain reaction. (PCR). Eleven different 16S rDNA genotypes were obtained from the combined data of RFLP analysis with four endonucleases and they were in agreement with the established taxonomic classification. Only one group of unclassified strains (Group 1) was assigned in a separate genotype, suggesting they belong to a new species. Using the 23S PCR RFLP method only six genotypes were detected, showing that this method is less discriminative than the 16S PCR-RFLP. Using the multilocus enzyme electrophoresis (MLEE) assay, the 48 strains tested could be classified into 35 zymovars. The seven enzymatic loci tested were polymorphic and the different profiles obtained among strains allowed the grouping of strains into 10 clusters. The PCR-RFLP methods together with the MLEE assay provide a rapid tool for the characterization and the establishment of the taxonomic position of isolates belonging to this nitrogen-fixing group, which shows a great potentiality in promoting plant growth. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.