Effects of endobronchial challenge with Actinobacillus pleuropneumoniae serotype 9 of pigs vaccinated with inactivated vaccines containing the Apx toxins
Effects of endobronchial challenge with Actinobacillus pleuropneumoniae serotype 9 of pigs vaccinated with inactivated vaccines containing the Apx toxins
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DOI：
10.1080/01652176.1998.9694841
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发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Haesebrouck, F
中科院分区：
文献类型：
--
作者：
Chiers, K;van Overbeke, I;Haesebrouck, F
测定了两种含有胸膜肺炎放线杆菌Apx毒素的灭活疫苗（HemopigTM，Biokema，洛桑，瑞士和PorcilisTM APP，Intervet，博克斯梅尔，荷兰）的效力。10头猪用Hemopig（TM）接种两次，8头猪用Porcilis（TM）App接种。10头对照动物用盐水注射两次。第二次接种后三周，所有猪支气管内接种10（5）个菌落形成单位（CFU）的A。胸膜肺炎血清型9菌株。攻毒后，在所有接种组和对照组猪中观察到呼吸频率增加和/或发热。Hemopig（TM）组和Porcilis（TM）App组的一头猪死亡，而对照组的所有猪在攻击中存活。攻毒后7天处死存活猪。对照组受影响肺组织的平均百分比为34%，Hemopig（TM）组为16%，Porcilis（TM）App组为17%。A.从所有10只对照动物的肺、从接种HemopigTM的10只动物中的7只动物和从接种PorcilisTM App的8只动物中的5只动物中分离出胸膜肺炎菌。对照组尾肺叶的平均细菌滴度为1.4 × 10（6）CFU/g，HemopigTM组为1.7 × 10（3）CFU/g，Porcilis（TM）App组为4.8x10（3）CFU/g。在两个接种组中，呼吸困难的平均天数、发热的平均天数、受累肺组织的平均百分比和肺尾叶的平均细菌滴度均显著低于对照组，两个接种组之间未观察到显著差异。得出的结论是，两种疫苗均诱导部分保护。
The efficacy of two inactivated vaccines containing the Apx toxins of Actinobacillus pleuropneumoniae (Hemopig(TM), Biokema, Lausanne, Switzerland and Porcilis(TM) APP, Intervet, Boxmeer, the Netherlands) was determined. Ten pigs were vaccinated twice with Hemopig(TM) and eight pigs with Porcilis(TM) App. Ten control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 10(5) colony-forming units (CFU) of an A. pleuropneumoniae serotype 9 strain. Increased respiratory rate and/or fever were observed in all vaccinated and control pigs after challenge. One pig of the Hemopig(TM) group and of the Porcilis(TM) App group died, whereas all pigs of the control group survived the challenge. Surviving pigs were killed at 7 days after challenge. The mean percentage of affected lung tissue was 34% in the control group, 16% in the Hemopig(TM) group, and 17% in the Porcilis(TM) App group. A. pleuropneumoniae was isolated from the lungs of all 10 control animals, from 7 of the 10 animals vaccinated with Hemopig(TM) and from 5 of the 8 animals vaccinated,vith Porcilis(TM) App. The mean bacterial titres of the caudal lung lobes were 1.4x10(6) CFU/g in the control group, 1.7x10(3) CFU/g in the Hemopig(TM) group, and 4.8x10(3) CFU/g in the Porcilis(TM) App group. In both vaccinated groups the mean number of days with dyspnoea, the mean number of days with fever, the mean percentage of affected lung tissue, and the mean bacterial titre in the caudal lung lobes were significantly lower than in the control group, Significant differences between the two vaccinated groups were not observed. It was concluded that both vaccines induced partial protection.