Pulsed-Field Gel Electrophoresis Analysis of Bordetella pertussis Isolates Circulating in Europe from 1998 to 2009
Pulsed-Field Gel Electrophoresis Analysis of Bordetella pertussis Isolates Circulating in Europe from 1998 to 2009
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DOI：
10.1128/jcm.02036-12
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发表时间：
2013-02-01
影响因子：
9.4
通讯作者：
He, Qiushui
中科院分区：
文献类型：
--
作者：
Advani, Abdolreza;Hallander, Hans O.;He, Qiushui
在1998年至2009年期间，在三个时期收集了百日咳博德特氏菌临床分离株，即，1998 - 2001年（n = 102）、2004 - 2005年（n = 154）和2007 - 2009年（n = 140），来自9个有不同疫苗接种计划的国家，即，丹麦、芬兰、法国、德国、荷兰、挪威、波兰、瑞典和英国。根据B流行病学分型的标准化建议进行脉冲场凝胶电泳（PFGE）分析。百日咳。有81种不同的PFGE谱，其中5种（BpSR 3、BpSR 5、BpSR 10、BpSR 11和BpSR 12）在396株分离株中的61%中观察到，并且在几乎所有国家中均占主导地位。主要特征BpSR 11呈下降趋势，从1998 - 2005年的25%-30%下降到2007 - 2009年的13%，BpSR 3和BpSR 10分别从0%和8%上升到21%和22%。这些图谱之间的一个差异是BpSR 11含有携带fim 3 -2等位基因的分离株，而BpSR 3和BpSR 10含有携带fim 3 -1等位基因的分离株。五个主要类别的总比例由1998至2001年的44%增至2004至2005年的63%，再增至2007至2009年的70%。总之，在B中鉴定了常见的PFGE图谱。在欧洲国家流行的百日咳人群，有不同的疫苗接种计划和不同的疫苗覆盖率。这些流行的分离株含有新的百日咳毒素启动子ptxP 3等位基因。然而，有证据表明ptxP 3菌株的特征在于不同的PFGE配置文件之间的多样化选择。这项工作表明，即使在10年的相对较短的时间跨度内，成功的分离株在欧洲传播并引起B的大的变化。可能出现百日咳菌群。
Between 1998 and 2009, Bordetella pertussis clinical isolates were collected during three periods, i.e., 1998 to 2001 (n = 102), 2004 to 2005 (n = 154), and 2007 to 2009 (n = 140), from nine countries with distinct vaccination programs, i.e., Denmark, Finland, France, Germany, The Netherlands, Norway, Poland, Sweden, and the United Kingdom. Pulsed-field gel electrophoresis (PFGE) analysis was performed according to standardized recommendations for epidemiological typing of B. pertussis. There were 81 different PFGE profiles, five of which (BpSR3, BpSR5, BpSR10, BpSR11, and BpSR12) were observed in 61% of the 396 isolates and shown to be predominant in almost all countries. The major profile, BpSR11, showed a decreasing trend from 25% to 30% in 1998 to 2005 to 13% in 2007 to 2009, and there were increases in BpSR3 and BpSR10 from 0% and 8% to 21% and 22%, respectively. One difference between these profiles is that BpSR11 contains isolates harboring the fim3-2 allele and BpSR3 and BpSR10 contain isolates harboring the fim3-1 allele. The total proportion of the five predominant profiles increased from 44% in 1998 to 2001 to 63% in 2004 to 2005 to 70% in 2007 to 2009. In conclusion, common PFGE profiles were identified in B. pertussis populations circulating in European countries with different vaccination programs and different vaccine coverages. These prevalent isolates contain the novel pertussis toxin promoter ptxP3 allele. However, there is evidence for diversifying selection between ptxP3 strains characterized by distinct PFGE profiles. This work shows that, even within a relatively short time span of 10 years, successful isolates which spread through Europe and cause large shifts in B. pertussis populations may emerge.