Analysis of cell growth in three-dimensional scaffolds
Analysis of cell growth in three-dimensional scaffolds
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DOI：
10.1089/ten.2006.12.705
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发表时间：
2006-04-01
影响因子：
--
通讯作者：
Wu, BM
中科院分区：
文献类型：
--
作者：
Dunn, JCY;Chan, WY;Wu, BM
从实验测量确定前成骨细胞在多层三维支架中的体外生长，并与数学模型进行比较。在细胞接种后，初始细胞密度在整个支架中是均匀的。10天后，流体-支架界面处的细胞密度从2.1 × 10（5）个细胞/cm（3）增加到1.3 × 10（7）个细胞/cm（3）。细胞密度的增加主要局限于离流体-支架界面最外的200 μ m。细胞密度分布与基于局部氧张力模拟细胞生长的数学模型非常一致。从这个数学模型得到的更好的理解可能在三维支架的设计中是有用的，可以支持更均匀的细胞生长。
The in vitro growth of pre-osteoblasts in multi-layer, three-dimensional scaffolds was determined from experimental measurements and was compared to a mathematical model. Immediately following cell seeding, the initial cell density was uniform throughout the scaffold. After 10 days, the cell density increased from 2.1 x 10(5) cells/cm(3) to 1.3 x 10(7) cells/cm(3) at the fluid-scaffold interface. The increase in cell density was largely confined to the outermost 200 mu m from the fluid-scaffold interface. The cell density profile was in good agreement with a mathematical model that simulated the cell growth based on the local oxygen tension. The improved understanding derived from this mathematical model may be useful in the design of three-dimensional scaffolds that can support more uniform growth of cells.