Effect of sodium dodecyl sulfate on polar lobe formation and function in Ilyanassa obsoleta embryos.
Effect of sodium dodecyl sulfate on polar lobe formation and function in Ilyanassa obsoleta embryos.
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十二烷基硫酸钠对 Ilyanassa obsoleta 胚胎极叶形成和功能的影响。
DOI：
10.1002/jez.1402530105
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Render,J
中科院分区：
文献类型：
--
作者：
Render,J
在前两次卵裂过程中，由Ilyanassa obsoletaembryos形成的无核植物极突起的极叶作为将形态发生决定因素分流到一个细胞的机制。极叶物质在第一次卵裂期间在CD细胞中分离，在第二次卵裂期间在D细胞中分离，导致非常不相等的四细胞阶段。幼虫的结构，包括外壳，脚，鳃盖，平衡囊，和眼睛的发展，只有当极叶材料。在第一次卵裂之前和期间用阴离子洗涤剂十二烷基硫酸钠（SDS）处理抑制极叶形成并均衡卵裂，因为叶材料分布到两个细胞中。SDS平衡的胚胎在第二次卵裂期间没有极叶形成，并且四细胞阶段由四个相等的细胞组成，细胞接触减少。SDS不可逆地抑制极叶形成，而不影响胞质分裂。尽管SDS平衡胚胎中27%的幼虫有一个或多个叶依赖性结构重复，但形态发生受损：超过40%的幼虫未能形成壳和/或平衡囊。当细胞在均衡的第一次卵裂后分离并成对饲养时，成对的幼虫以与整个均衡胚胎相同的频率复制叶依赖性结构。SDS处理的胚胎中受损的形态发生的可能解释进行了讨论。
Polar lobes, anucleate vegetal pole protrusions formed byIlyanassa obsoletaembryos, serve as a mechanism for shunting morphogenetic determinants to one cell during the first two cleavages. Polar lobe material becomes segregated in the CD cell during first cleavage and in the D cell during second cleavage, resulting in a very unequal four‐cell stage. Larval structures including external shell, foot, operculum, statocysts, and eyes develop only when polar lobe material is present. Treatment with the anionic detergent sodium dodecyl sulfate (SDS) before and during the first cleavage inhibited polar lobe formation and equalized cleavage, as the lobe material was distributed to two cells. No polar lobes formed during second cleavage in SDS‐equalized embryos, and the four‐cell stage consisted of four equal cells with reduced cell contacts. SDS irreversibly inhibited polar lobe formation without affecting cytokinesis. Although 27% of the larvae from SDS‐equalized embryos had one or more lobe‐dependent structures duplicated, morphogenesis was impaired: more than 40% of such larvae failed to form shell and/or statocysts. When cells were separated after equalized first cleavage and raised as pairs, the pairs of resulting larvae duplicated lobe‐dependent structures with the same frequency as whole equalized embryos. Possible explanations for impaired morphogenesis in SDS‐treated embryos are discussed.