A systemic lupus erythematosus gene expression array in disease diagnosis and classification: a preliminary report.
A systemic lupus erythematosus gene expression array in disease diagnosis and classification: a preliminary report.
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DOI：
10.1177/0961203310383072
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发表时间：
2011-03
期刊：
影响因子：
2.6
通讯作者：
Tsokos GC
中科院分区：
文献类型：
--
作者：
Juang YT;Peoples C;Kafri R;Kyttaris VC;Sunahori K;Kis-Toth K;Fitzgerald L;Ergin S;Finnell M;Tsokos GC
系统性红斑狼疮 (SLE) 是一种临床异质性疾病，根据一系列临床和实验室检查结果进行诊断。在致病水平上，已经认识到使用不同生化和分子途径的多种因素。临床疾病子集的简洁识别和分类以及疾病生物标志物的可用性在很大程度上仍未得到解决。根据本作者和其他实验室提供的信息，开发了一种由 30 个基因组成的狼疮基因表达阵列，之前声称这些基因与 T 细胞的异常功能有关。另外八个基因被纳入作为对照。外周血取自 10 名 SLE 患者（19 个样本）、6 名类风湿性关节炎 (RA) 患者以及 19 名健康对照者。对T细胞mRNA进行反转录和PCR，并测量基因表达水平。常规统计分析与主成分分析（PCA）一起进行，以捕获所有基因对疾病诊断和临床参数的贡献。狼疮基因表达阵列忠实地反映了基因的表达水平。使用相对少量（5ml）的外周血记录的表达变化反映了文献中报道的变化。基因表达水平的 PCA 将 SLE 样本与正常和 RA 样本区分开来，无论疾病活动如何。各个主要成分倾向于定义特定的疾病表现，例如关节炎和蛋白尿。因此，基于之前声称有助于 SLE 免疫发病机制的基因的狼疮基因表达阵列可以定义该疾病，并且 30 个基因表达的主要成分可以定义具有特定疾病表现的患者。
Systemic lupus erythematosus (SLE) is a clinically heterogeneous disease diagnosed on the presence of a constellation of clinical and laboratory findings. At the pathogenetic level, multiple factors using diverse biochemical and molecular pathways have been recognized. Succinct recognition and classification of clinical disease subsets, as well as the availability of disease biomarkers, remains largely unsolved. Based on information produced by the present authors’ and other laboratories, a lupus gene expression array consisting of 30 genes, previously claimed to contribute to aberrant function of T cells, was developed. An additional eight genes were included as controls. Peripheral blood was obtained from 10 patients (19 samples) with SLE and six patients with rheumatoid arthritis (RA) as well as 19 healthy controls. T cell mRNA was subjected to reverse transcription and PCR, and the gene expression levels were measured. Conventional statistical analysis was performed along with principal component analysis (PCA) to capture the contribution of all genes to disease diagnosis and clinical parameters. The lupus gene expression array faithfully informed on the expression levels of genes. The recorded changes in expression reflect those reported in the literature by using a relatively small (5ml) amount of peripheral blood. PCA of gene expression levels placed SLE samples apart from normal and RA samples regardless of disease activity. Individual principal components tended to define specific disease manifestations such as arthritis and proteinuria. Thus, a lupus gene expression array based on genes previously claimed to contribute to immune pathogenesis of SLE may define the disease, and principal components of the expression of 30 genes may define patients with specific disease manifestations.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
13.6
作者：
Crispín JC;Liossis SN;Kis-Toth K;Lieberman LA;Kyttaris VC;Juang YT;Tsokos GC
通讯作者：
Tsokos GC
狼疮中的 T 细胞和 B 细胞信号传导生物标志物和治疗靶点。
影响因子：
5.1
作者：
Perl A;Fernandez DR;Telarico T;Doherty E;Francis L;Phillips PE
通讯作者：
Phillips PE
影响因子：
3.5
作者：
Perl A
通讯作者：
Perl A
影响因子：
4.4
作者：
Solomou, EE;Juang, YT;Tsokos, GC
通讯作者：
Tsokos, GC