Combined effects of exercise training and D-allulose intake on endurance capacity in mice.
Combined effects of exercise training and D-allulose intake on endurance capacity in mice.
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DOI：
10.14814/phy2.15297
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发表时间：
2022-05
影响因子：
2.5
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中科院分区：
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本研究探讨了运动训练和D-大豆糖摄入对小鼠耐力的影响。将雄性C57BL/6J小鼠分为安静(SED)组和运动训练组(分别为CON-SED组、CON-Ex组、allu-SED组、allu-Ex组，n=6~7个/组)。EX组的小鼠每周在电动跑步机上训练5天，为期4周(15-18米/分钟，60米/分钟)。运动训练结束后，所有小鼠都接受了一次力竭跑步测试，以评估它们的耐力。运动后48h，各给药组小鼠再次以18m/min的速度跑步60min。运动后即刻取腓肠肌和肝脏标本。直到精疲力竭为止，allu-Ex组的运行时间往往比CON-Ex组的运行时间更长(p=0.08)。Con-Ex组肌糖原含量显著低于Con-SED组，allu-Ex组显著高于Con-Ex组(P<0.05)。此外，运动训练增加了肌肉和肝脏中一磷酸腺苷活化蛋白激酶(AMPK)的磷酸化水平。肌肉和肝脏中AMPK下游的乙酰辅酶A羧基酶(ACC)的磷酸化水平在Alu-Ex组显著高于ConSED组(p<0.05)，提示运动训练和D-大豆糖的结合可能激活了AMPK-ACC信号通路，这与肌肉和肝脏中的脂肪酸氧化有关。综上所述，我们的数据表明，运动训练和D-大豆糖摄入相结合是提高小鼠耐力的有效策略。这可能与减少骨骼肌糖原含量和增强AMPK-ACC信号的激活有关。本研究探讨了运动训练和D-大豆糖摄入对小鼠耐力的影响。我们的数据表明，运动训练和D-大豆糖摄入相结合是一种有效的策略，通过增强脂肪代谢和节省骨骼肌糖原含量来提高耐力。
This study investigated the combined effects of exercise training and D‐allulose intake on endurance capacity in mice. Male C57BL/6J mice were fed either a control diet (Con) or a 3% D‐allulose diet (Allu) and further divided into the sedentary (Sed) or exercise training (Ex) groups (Con‐Sed, Con‐Ex, Allu‐Sed, Allu‐Ex, respectively; n = 6–7/group). The mice in the Ex groups were trained on a motor‐driven treadmill 5 days/week for 4 weeks (15–18 m/min, 60 min). After the exercise training period, all mice underwent an exhaustive running test to assess their endurance capacity. At 48 h after the running test, the mice in the Ex groups were subjected to run at 18 m/min for 60 min again. Then the gastrocnemius muscle and liver were sampled immediately after the exercise bout. The running time until exhaustion tended to be higher in the Allu‐Ex than in the Con‐Ex group (p = 0.08). The muscle glycogen content was significantly lower in the Con‐Ex than in the Con‐Sed group and was significantly higher in the Allu‐Ex than in the Con‐Ex group (p < 0.05). Moreover, exercise training increased the phosphorylation levels of adenosine monophosphate‐activated protein kinase (AMPK) in the muscle and liver. The phosphorylation levels of acetyl coenzyme A carboxylase (ACC), a downstream of AMPK, in the muscle and liver were significantly higher in the Allu‐Ex than in the Con‐Sed group (p < 0.05), suggesting that the combination of exercise training and D‐allulose might have activated the AMPK‐ACC signaling pathway, which is associated with fatty acid oxidation in the muscle and liver. Taken together, our data suggested the combination of exercise training and D‐allulose intake as an effective strategy to upregulate endurance capacity in mice. This may be associated with sparing glycogen content and enhancing activation of AMPK‐ACC signaling in the skeletal muscle. This study investigated the combined effects of exercise training and D‐allulose intake on endurance capacity in mice. Our data suggest the combination of exercise training and D‐allulose intake as an effective strategy to upregulate endurance capacity by enhancing lipid metabolism and sparing glycogen content in the skeletal muscle.