Effects of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol on parameters associated with oxidative stress in HepG2 cells
Effects of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol on parameters associated with oxidative stress in HepG2 cells
复制标题
DOI：
10.1007/s12550-019-00344-0
复制
发表时间：
2019-05-01
影响因子：
3
通讯作者：
Ruiz, Maria-Jose
中科院分区：
文献类型：
--
作者：
Juan-Garcia, Ana;Juan, Cristina;Ruiz, Maria-Jose
这项工作研究了脱氧雪腐镰刀菌烯醇 (DON) 及其两种乙酰化类似物（3-ADON、15-ADON）对 HepG2 细胞中氧化应激状态第一个指标的影响，即活性氧 (ROS) 的产生和脂质过氧化 (LPO) 的诱导。 HepG2 细胞单独或组合暴露于不同浓度的三种毒素中 24、48 和 72 小时。 HepG2细胞中获得的细胞毒性结果与ROS和LPO的检测相关。对于个体治疗中检测的霉菌毒素，这种效应与 ROS 呈负相关，而与 LPO 呈正相关。含有 15-ADON 的两种毒素的组合产生最高值，而含有 3-ADON 的两种毒素组合的影响较小。所有三种霉菌毒素的组合都会减轻 ROS 的产生，并且检测到 LPO 水平最高，这与 HepG2 早期产生的 ROS 适应的最终崩溃有关。总之，本研究中提出的应激评估参数（ROS 和 LPO）揭示了单独或组合暴露于 DON、3-ADON 和 15-ADON 的 HepG2 细胞的增加。
This work studied the effect of deoxynivalenol (DON) and two of its acetylated analogs (3-ADON, 15-ADON) on first indicators of oxidative stress status, namely production of reactive oxygen species (ROS) and induction of lipid peroxidation (LPO), in HepG2 cells. HepG2 cells were exposed to different concentrations of the three toxins, either alone or in combinations, for 24, 48, and 72h. Results of cytotoxicity obtained in HepG2 cells were correlated with the detection of ROS and LPO. This effect was inversely correlated with ROS while directly correlated with LPO for the assayed mycotoxins in individual treatment. Combinations of two toxins containing 15-ADON yielded highest values, while for two-toxin combinations with 3-ADON, the effects were minor. A combination of all three mycotoxins alleviated ROS production and the highest levels in LPO were detected, in association to a final breakdown of adaption of ROS early produced by HepG2. In conclusion, parameters of stress evaluation presented in this study (ROS and LPO), revealed increases in HepG2 cells exposed to DON, 3-ADON, and 15-ADON either individually or combined.