THE 5' UNTRANSLATED REGION OF THE I FACTOR, A LONG INTERSPERSED NUCLEAR ELEMENT-LIKE RETROTRANSPOSON OF DROSOPHILA-MELANOGASTER, CONTAINS AN INTERNAL PROMOTER AND SEQUENCES THAT REGULATE EXPRESSION
THE 5' UNTRANSLATED REGION OF THE I FACTOR, A LONG INTERSPERSED NUCLEAR ELEMENT-LIKE RETROTRANSPOSON OF DROSOPHILA-MELANOGASTER, CONTAINS AN INTERNAL PROMOTER AND SEQUENCES THAT REGULATE EXPRESSION
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DOI：
10.1128/mcb.13.2.1042
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发表时间：
1993-02-01
影响因子：
5.3
通讯作者：
FINNEGAN, DJ
中科院分区：
文献类型：
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作者：
MCLEAN, C;BUCHETON, A;FINNEGAN, DJ
黑腹果蝇杂种发育不全的I-R系统是由一个长散布的核元件样逆转座子I因子控制的。转座的I因子发生在一个高的频率，只有在卵巢的女性杂交雄性诱导株，含有功能性I因子与女性的反应株，缺乏它们。在这项研究中，将I因子的5'非翻译区连接到氯霉素乙酰转移酶基因上，并在转染的D.黑腹组织培养细胞和转化的果蝇。结果已经鉴定了位于I因子的前186 bp内的启动子。缺失分析表明，核苷酸+1至+40对于高启动子活性和准确的转录起始是足够的。该区域包含在一类RNA聚合酶II启动子中发现的序列，其缺乏TATA盒和富含CpG的基序。在转化的苍蝇，高水平的表达，从核苷酸+1至+186被限制在卵巢的反应性女性，这表明该启动子参与组织和细胞型特异性的转座。
The I-R system of hybrid dysgenesis in Drosophila melanogaster is controlled by a long interspersed nuclear element-like retroposon, the I factor. Transposition of the I factor occurs at a high frequency only in the ovaries of females produced by crossing males of inducer strains that contain functional I factors with females of reactive strains that lack them. In this study, the 5' untranslated region of the I factor was joined to the chloramphenicol acetyltransferase gene, and activity was assayed in transfected D. melanogaster tissue culture cells and transformed flies. The results have identified a promoter that lies within the first 186 bp of the I factor. Deletion analysis shows that nucleotides + 1 to + 40 are sufficient for high promoter activity and accurate transcription initiation. This region contains sequences that are found in a class of RNA polymerase II promoters that lack both a TATA box and CpG-rich motifs. In transformed flies, high levels of expression from nucleotides + 1 to + 186 are confined to the ovaries of reactive females, suggesting that the promoter is involved in the tissue and cytotype specificity of transposition.