Observation of T-2 toxin and HT-2 toxin glucosides from Fusarium sporotrichioides by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
Observation of T-2 toxin and HT-2 toxin glucosides from Fusarium sporotrichioides by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS).
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DOI：
10.3390/toxins3121554
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发表时间：
2011-12
期刊：
影响因子：
4.2
通讯作者：
Maragos CM
中科院分区：
文献类型：
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作者：
Busman M;Poling SM;Maragos CM
采用高效液相色谱-串联质谱（LC-MS/MS）对分枝镰刀菌固体和液体培养物产生的单端孢霉烯族化合物进行了评估。除了预期的 T-2 毒素 HT-2 毒素和新茄醇之外，还检测到了另外两种化合物，其离子比 T-2 毒素或 HT-2 毒素的质谱中的离子高 162 m/z。这两种化合物的断裂行为与T-2毒素和HT-2毒素相似。基于LC-MS/MS行为，推测这两种化合物是T-2毒素3-O-葡萄糖苷和HT-2毒素3-O-葡萄糖苷。在接种了分枝孢镰刀菌的小麦和燕麦籽粒中，以及在液体培养基中和破碎的玉米或大米上生长的培养物中，测量了这两种糖苷的产量。小麦和燕麦中产生的糖苷表明它们也可能存在于自然污染的谷物中。
The trichothecenes produced by solid and liquid cultures of Fusarium sporotrichioides were evaluated with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Along with the expected T-2 toxin HT-2 toxin and neosolaniol, two additional compounds were detected, which had ions 162 m/z higher than those in the mass spectra of T-2 toxin or HT-2 toxin. Fragmentation behavior of these two compounds was similar to that of T-2 toxin and HT-2 toxin. Based on LC-MS/MS behavior, it is proposed that the two compounds are T-2 toxin 3-O-glucoside and HT-2 toxin 3-O-glucoside. Production of the two glucosides was measured in kernels from wheat and oat inoculated with F. sporotrichiodes, as well as in cultures grown in liquid media and on cracked corn or rice. Production of glucosides in wheat and oats suggest that they may also be present in naturally contaminated cereals.