Transformation of amorphous calcium carbonate to rod-like single crystal calcite via "copying" collagen template.
Transformation of amorphous calcium carbonate to rod-like single crystal calcite via "copying" collagen template.
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DOI：
10.1016/j.msec.2015.05.079
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发表时间：
2015-10
期刊：
影响因子：
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通讯作者：
Zhonghui Xue;Bin-bin Hu;S. Dai;Z. Du
中科院分区：
文献类型：
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作者：
Zhonghui Xue;Bin-bin Hu;S. Dai;Z. Du
通过将胶原溶液铺展在去离子水、CaCl 2 溶液和Ca(HCO 3 ) 2 溶液上来制备胶原朗缪尔膜。然后将所得胶原朗缪尔单层压缩至 10 mN/m 的侧向压力并保持不同的持续时间，从而使 CaCO3 结晶。研究了结晶时间对CaCO3物相组成和微观结构的影响。结果发现，结晶时间为 6 小时即可获得无定形碳酸钙 (ACC)。通过“复制”胶原纤维的对称性和维度，在 12 小时和 24 小时的延长结晶时间下，无定形 CaCO3 转化为棒状单晶方解石晶体。所得方解石微晶沿胶原纤维的纵轴良好定向。胶原纤维的有序表面结构和静电相互作用在调节方解石微晶的定向成核和生长方面发挥着关键作用。矿化胶原同时具有胶原纤维所需的机械性能和碳酸钙良好的生物相容性，可以组装成理想的骨植入生物材料。
Collagen Langmuir films were prepared by spreading the solution of collagen over deionized water, CaCl2solution and Ca(HCO3)2solution. Resultant collagen Langmuir monolayers were then compressed to a lateral pressure of 10 mN/m and held there for different duration, allowing the crystallization of CaCO3. The effect of crystallization time on the phase composition and microstructure of CaCO3was investigated. It was found that amorphous calcium carbonate (ACC) was obtained at a crystallization time of 6 h. The amorphous CaCO3was transformed to rod-like single crystal calcite crystals at an extended crystallization time of 12 h and 24 h, via “copying” the symmetry and dimensionalities of collagen fibers. Resultant calcite crystallites were well oriented along the longitudinal axis of collagen fibers. The ordered surface structure of collagen fibers and electrostatic interactions played key roles in tuning the oriented nucleation and growth of the calcite crystallites. The mineralized collagen possessing both desired mechanical properties of collagen fiber and good biocompatibility of calcium carbonate may be assembled into an ideal biomaterial for bone implants.