Modification of the structure and activity of lipid A in Yersinia pestis lipopolysaccharide by growth temperature
Modification of the structure and activity of lipid A in Yersinia pestis lipopolysaccharide by growth temperature
复制标题
生长温度改变鼠疫耶尔森菌脂多糖中脂质 A 的结构和活性
DOI：
10.1128/iai.70.8.4092-4098.2002
复制
发表时间：
2002-08-01
影响因子：
3.1
通讯作者：
Matsuura, M
中科院分区：
文献类型：
--
作者：
Kawahara, K;Tsukano, H;Matsuura, M
采用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法对鼠疫Yeisinia pestis Yreka菌株在27℃和37℃条件下的脂质A结构（脂质A-27℃和脂质A-37℃）进行了研究。脂质a- 27度c由三酰基、四酰基、五酰基和六酰基脂质a组成，其中四酰基脂质a含量最多。脂质A-37°c主要由三酰基和四酰基分子组成，只有少量的五酰基脂质A；六。检测-酰基脂质A。此外，脂质a -27°c中4-氨基阿拉伯糖的含量明显高于脂质a -37°c。利用小鼠和人巨噬细胞细胞系，通过诱导肿瘤坏死因子α (tnf - α)的能力来测定LPS和脂质A制剂的生物活性。在两种细胞系中，27℃培养的细菌的LPS和脂质A诱导TNF-a的作用强于37℃培养的细菌。然而，在人巨噬细胞中，活性差异更为明显。这些结果表明，为了降低人巨噬细胞的活化，在37℃下产生低酰化的脂质A可能对鼠疫杆菌更有利。
Yeisinia pestis strain Yreka was grown at 27 or 37degreesC, and the lipid A structures (lipid A-27degreesC and lipid A-37degreesC) of the respective lipopolysaccharides (LPS) were investigated by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. Lipid A-27degreesC consisted of a mixture of tri-acyl, tetra-acyl, penta-acyl, and hexa-acyl lipid A's, of which tetra-acyl lipid A was most abundant. Lipid A-37degreesC consisted predominantly of tri- and tetra-acylated molecules, with only small amounts of penta-acyl lipid A; no hexa.-acyl lipid A was detected. Furthermore, the amount of 4-amino-arabinose was substantially higher in lipid A-27degreesC than in lipid A-37degreesC. By use of mouse and human macrophage cell lines, the biological activities of the LPS and lipid A preparations were measured via their abilities to induce production of tumor necrosis factor alpha (TNF-alpha). In both cell lines the LPS and the lipid A from bacteria grown at 27degreesC were stronger inducers of TNF-a than those from bacteria grown at 37degreesC. However, the difference in activity was more prominent in human macrophage cells. These results suggest that in order to reduce the activation of human macrophages, it may be more advantageous for Y. pestis to produce less-acylated lipid A at 37degreesC.