Inactivation of some bacterial and animal viruses by exposure to liquid-air interfaces.
Inactivation of some bacterial and animal viruses by exposure to liquid-air interfaces.
复制标题
通过暴露于液-气界面使一些细菌和动物病毒失活。
DOI：
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发表时间：
1974
影响因子：
3.8
通讯作者：
A. Plantinga
中科院分区：
文献类型：
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作者：
T. Trouwborst;S. Kuyper;J. C. D. Jong;A. Plantinga
摘要
对EMC病毒和塞姆利基森林病毒的T1、T3、T5、MS2噬菌体表面灭活进行了研究，将病毒暴露在大的空气/水界面上，通过曝气或在球形烧瓶中旋转液体。EMC病毒在1m-Nacl溶液中对该处理不敏感，对噬菌体T3和T5影响不大，但T1和MS2噬菌体和Semliki森林病毒可通过悬浮液鼓泡空气或氮气迅速灭活。在静止的盐溶液中，没有观察到这些病毒的灭活。通过添加蛋白胨、非极性羧酸和表面活性剂OED，可以防止因曝气而失活。如果存在较大的溶液/玻璃界面，病毒会通过吸附到玻璃表面而损失一些。苯丙氨酸可防止吸附到玻璃表面，但不能有效防止曝气使其失活。表面失活速率强烈依赖于介质中的盐浓度。在低浓度的NaC l(0.01m)下，噬菌体T1和MS2几乎没有失活，而噬菌体T3对曝气不敏感，但在2.6m-NaC l中迅速失活。灭活速率随着摇动时间的延长而降低，在噬菌体T1的情况下，仍有10-4的原始颗粒几乎完全抵抗。对表面失活的抗性是一种非遗传特性。对热失活的抗性与对表面失活的抗性不相关，这表明在这两个过程中失活的机制是不同的。
Summary
Surface inactivation of the bacteriophages T1, T3, T5, MS2, of EMC virus and Semliki Forest virus was studied, exposing the viruses to a large air/water interface by aeration or by rotating the fluid in a spherical flask. EMC virus in 1 m-NaCl was not sensitive to this treatment, phage T3 and T5 were only little affected, but the phages T1 and MS2 and Semliki Forest virus were rapidly inactivated by bubbling air or nitrogen gas through the suspension. In salt solutions at rest no inactivation of these viruses was observed. Inactivation by aeration was prevented by addition of peptone, by apolar carboxylic acids and by the surface active agent OED. If a large solution/glass interface is present, some loss of virus occurs by adsorption to the glass surface. Phenylalanine protected against adsorption to the glass surface, but protected less effectively against inactivation by aeration. The rate of surface inactivation was strongly dependent on the salt concentration in the medium. At low NaCl concentration (0.01 m) nearly no inactivation was found for phage T1 and MS2 and phage T3 was not sensitive to aeration in 1 m-NaCl but was rapidly inactivated in 2.6 m-NaCl. The rate of inactivation decreased with time of shaking and in the case of phage T1 a nearly completely resistant fraction of 10-4 of the original particles remained. The resistance against surface inactivation was a non-heritable property. Resistance against thermal inactivation was not correlated with resistance to surface inactivation, suggesting that the mechanism of inactivation differs in these processes.