Proteome Analysis of Temporomandibular Joint with Disc Displacement
Proteome Analysis of Temporomandibular Joint with Disc Displacement
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伴有椎间盘移位的颞下颌关节的蛋白质组分析
DOI：
10.1177/00220345221110099
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发表时间：
2022-10-20
影响因子：
7.6
通讯作者：
Yang, C.
中科院分区：
文献类型：
--
作者：
Liu, X.;Yang, Y.;Yang, C.
关节盘无复位移位是一种常见的颞下颌关节紊乱病，会引起临床症状，有时还会导致髁状突退变。在某些情况下，在椎间盘重定位过程中可以检测到骨再生。然而，到目前为止，关于关节盘位置与髁状突结果的蛋白水平的系统范围的知识仍然缺乏。在这里，我们使用独立于数据的高分辨率采集质谱仪对109名没有复位的关节盘移位患者的滑液进行了全面的表达谱分析，并表征了1,714个蛋白质的差异。根据磁共振成像，样本被分成有无髁突吸收组和有无新生骨的亚组。在蛋白质组学分析中，有32个蛋白质组具有统计学意义(2倍，P<；0.05)。与非骨吸收组丰富的体液调节、囊泡转运和局部黏附等途径相比，骨吸收组中对无机物质的反应、凝血和雌激素信号转导等途径的表达显著增强。在亚组分析中，45个有重要意义的蛋白质(>；2倍，P<；0.05)与Will愈合、糖酵解和糖异生以及氨基酸代谢等途径相关。结合临床检查，乙酰辅酶A羧基酶β(ACACB)和转化生长因子β1(TGFB1)等分子与视觉模拟评分和最大切迹开度等指标相关(P<；0.05)。免疫印迹法检测孕酮免疫调节结合因子1(PIBF1)、富含组氨酸的糖蛋白(HRG)、蛋白激酶C和酪蛋白激酶底物2(PACSIN2)等7种蛋白的表达。综上所述，这项研究首次对关节盘移位时无复位时的髁状突吸收和关节盘复位后的新骨形成进行了蛋白质组学分析。结合临床数据，这一分析提供了一个重要的洞察力，在关节盘位置的髁突修改的蛋白质组学。
Disc displacement without reduction is a common disorder of the temporomandibular joint, causing clinical symptoms and sometimes condylar degeneration. In some cases, bone regeneration is detected following disc-repositioning procedures. Until now, however, systems-wide knowledge of the protein levels for condylar outcome with disc position is still lacking. Here, we performed comprehensive expression profiling of synovial fluid from 109 patients with disc displacement without reduction using high-resolution data-independent acquisition mass spectrometry and characterized differences in 1,714 proteins. Based on magnetic resonance imaging, samples were divided into groups with versus without condylar absorption and subgroups with versus without new bone. For the proteomic analysis, 32 proteins in groups presented with statistical significance (>2-fold, P < 0.05). Pathways such as response to inorganic substances, blood coagulation, and estrogen signaling were significantly expressed in the group with bone absorption as compared with pathways such as regulation of body fluid levels, vesicle-mediated transport, and focal adhesion, which were enriched in the group without bone absorption. In subgroup analysis, 45 proteins of significant importance (>2-fold, P < 0.05) were associated with pathways including would healing, glycolysis and gluconeogenesis, and amino acid metabolism. Combined with clinical examination, molecules such as acetyl-CoA carboxylase beta (ACACB) and transforming growth factor beta 1 (TGFB1) were related to features such as visual analog scale and maximum interincisal opening (P < 0.05). In addition, 7 proteins were examined by Western blotting, including progesterone immunomodulatory binding factor 1 (PIBF1), histidine-rich glycoprotein (HRG), and protein kinase C and casein kinase substrate in neurons 2 (PACSIN2). In conclusion, this study provides the first proteome analysis of condylar absorption at disc displacement without reduction and postoperative new bone formation after disc reposition. Integrated with clinical data, this analysis provides an important insight into the proteomics of condylar modification at disc position.