Molecular studies on the ouabain binding site of the Na+, K+-ATPase in milkweed butterflies
Molecular studies on the ouabain binding site of the Na+, K+-ATPase in milkweed butterflies
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DOI：
10.1007/pl00001823
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发表时间：
2000-01-01
期刊：
影响因子：
1.8
通讯作者：
Schneider, M
中科院分区：
文献类型：
--
作者：
Mebs, D;Zehner, R;Schneider, M
黑脉金斑蝶（Danaus plexippus）的Na (+), K (+) - atp酶对心脏糖苷的抑制不敏感，这是由于在代表瓦巴因结合位点的a亚基的122位上替换了组氨酸而不是天冬酰胺。通过对该位点的DNA序列进行PCR扩增，从丹纳科（Dananus plexippus、d.c chrysippus、d.g illilipus、d.p ilene、d.c ilene、d.c genutia、Tirumala hamata、Euploea spp.、Parantica weiskei、P.melusine）、鞘科（Daphnis nerii）和拟乳草蝶（hyppolimnas missipus、Limenitis archippus和l.a arthemis, Nymphalidae）中提取的DNA中获得了270 bp的PCR产物。核苷酸序列分析显示，瓦巴因结合域（CAC-His的AAC-Asn）的单点突变只存在于Danaus plexippus中，而不存在于其他物种中。由于这些马利筋蝴蝶也储存果仁内酯，Na+， K+- atp酶的其他结构修饰可能已经发生，或者已经形成了其他的果仁内酯耐受性策略。
The Na (+), K (+) -ATPase of the Monarch butterfly (Danaus plexippus) is insensitive to the inhibition by cardiac glycosides due to an amino acid replacement: histidine instead of asparagine at position 122 of the a-subunit representing the ouabain binding site. By PCR amplification of the DNA sequence of this site, a PCR product of 270 bp was obtained from DNA extracted from Danainae species (Dananus plexippus, D.chrysippus, D.gillipus, D.philene, D.genutia, Tirumala hamata, Euploea spp., Parantica weiskei, P.melusine), Sphingidae (Daphnis nerii) and mimics of milkweed butterflies (Hypolimnas missipus, Limenitis archippus and L.arthemis, Nymphalidae). Analysis of the nucleotide sequences revealed that the single point mutation in the ouabain binding domain (AAC-Asn for CAC-His) was present only in Danaus plexippus, but not in the other species investigated. Since these milkweed butterflies also store cardenolides, other structural modifications of the Na+, K+-ATPase may have occurred or other strategies of cardenolide tolerance have been developed.