Sequence-specific, Golgi-dependent vacuolar targeting of castor bean 2S albumin.
Sequence-specific, Golgi-dependent vacuolar targeting of castor bean 2S albumin.
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蓖麻子 2S 白蛋白的序列特异性、高尔基体依赖性液泡靶向。
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
L. Roberts
中科院分区：
文献类型：
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作者：
J. Brown;N. A. Jolliffe;L. Frigerio;L. Roberts
通过在转化的烟草（Nicotiana tabacum）叶细胞中表达cDNA并通过在天然组织中进行生物合成来研究蓖麻子（Ricinus communis）2S白蛋白前体的靶向。两种组织中的正确靶向伴随着前体的处理。交付到液泡是敏感的布雷菲德菌素A（BFA）治疗在这两个组织和扰动COPII功能在烟草，支持通过高尔基体的运输是必需的。这种高尔基体依赖性路由的靶向信号位于前白蛋白的第一异源二聚体的前肽内。该前肽在体内以高尔基体依赖的方式将正常分泌的报告蛋白导向烟草细胞的液泡，除非关键的Leu残基发生突变，这支持了需要序列特异性信号将种子贮藏蛋白导向营养细胞的液泡的观点。
The targeting of the castor bean (Ricinus communis) 2S albumin precursor has been investigated by expressing cDNA in transformed tobacco (Nicotiana tabacum) leaf cells and by following biosynthesis in the native tissue. Correct targeting in both tissues was accompanied by processing of the precursor. Delivery to vacuoles was sensitive to brefeldin A (BFA) treatment in both tissues and to perturbation of COPII function in tobacco, supporting the view that transport through the Golgi is required. The targeting signal for this Golgi-dependent routing lies within the propeptide of the first heterodimer of proalbumin. This propeptide directed a normally secreted reporter protein to the vacuoles of tobacco cells in a Golgi-dependent manner in vivo, unless a critical Leu residue was mutated, supporting the view that a sequence-specific signal was needed to target a seed storage protein to the vacuoles of a vegetative cell.