Quantitative analysis of polarity in 3D reveals local cell coordination in the embryonic mouse heart
Quantitative analysis of polarity in 3D reveals local cell coordination in the embryonic mouse heart
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DOI：
10.1242/dev.087940
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发表时间：
2013-01-15
期刊：
影响因子：
4.6
通讯作者：
Meilhac, Sigolene M.
中科院分区：
文献类型：
--
作者：
Le Garrec, Jean-Francois;Ragni, Chiara V.;Meilhac, Sigolene M.
器官形态发生的基础各向异性已经在2D中被量化，利用了参考轴的优势。然而，形态发生是一个3D过程，在3D中分析细胞的极性仍然是一个挑战。在这里，我们设计了一种新的过程，集成了多学科工具，包括图像分割、统计分析、轴向聚类和相关分析。其结果是与随机的轴向分布相比，对3D中细胞取向的显著排列进行了敏感和公正的评估。以小鼠心脏为模型，我们在已知心肌细胞排列的胎儿阶段验证了这一过程。在胚胎阶段，我们的研究表明，心室细胞已经在局部协调。中心体-核轴和细胞分裂轴在与心脏外表面平行的平面上偏向，有少量的跨壁成分。我们显示了这些轴在心脏表面平面上的进一步对齐。我们的方法一般适用于3D组织中的其他矢量或轴集合，以绘制它们显示显著比对的区域。
Anisotropies that underlie organ morphogenesis have been quantified in 2D, taking advantage of a reference axis. However, morphogenesis is a 3D process and it remains a challenge to analyze cell polarities in 3D. Here, we have designed a novel procedure that integrates multidisciplinary tools, including image segmentation, statistical analyses, axial clustering and correlation analysis. The result is a sensitive and unbiased assessment of the significant alignment of cell orientations in 3D, compared with a random axial distribution. Taking the mouse heart as a model, we validate the procedure at the fetal stage, when cardiomyocytes are known to be aligned. At the embryonic stage, our study reveals that ventricular cells are already coordinated locally. The centrosome-nucleus axes and the cell division axes are biased in a plane parallel to the outer surface of the heart, with a minor transmural component. We show further alignment of these axes locally in the plane of the heart surface. Our method is generally applicable to other sets of vectors or axes in 3D tissues to map the regions where they show significant alignment.