Irradiated Pancreatic Cancer Cells Undergo both Apoptosis and Necrosis, and Could Be Phagocytized by Dendritic Cells
Irradiated Pancreatic Cancer Cells Undergo both Apoptosis and Necrosis, and Could Be Phagocytized by Dendritic Cells
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DOI：
10.1159/000087868
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发表时间：
2005-07
影响因子：
1.6
通讯作者：
H. Shimamura;M. Sunamura;K. Tsuchihara;S. Egawa;K. Takeda;S. Matsuno
中科院分区：
文献类型：
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作者：
H. Shimamura;M. Sunamura;K. Tsuchihara;S. Egawa;K. Takeda;S. Matsuno
研究了未成熟树突状细胞（DC）与辐照胰腺癌细胞的相互作用。使用膜联蛋白V和碘化丙啶的流式细胞术分析显示，电离辐射（25-35戈伊X射线）诱导胰腺癌细胞系的凋亡和坏死。照射后，PK-1和Panc-1细胞可能发生坏死，而MIAPaCa-2细胞发生凋亡。当DiO染色的未成熟DC与DiI染色的辐射MIAPaCa-2共孵育时，在荧光显微镜下观察到DC早在共孵育后4小时就吞噬死亡的肿瘤细胞。流式细胞仪检测证实了DC对照射后肿瘤细胞的吞噬作用。这些结果表明，经过辐射的胰腺癌细胞会发生细胞凋亡和坏死，可能是DC交叉提呈的肿瘤相关抗原的良好来源。
The interaction of immature dendritic cells (DC) with irradiated pancreatic cancer cells was examined. Flow cytometric analysis using annexin V and propidium iodide revealed that ionizing radiation (25–35 Gy X-ray) induced both apoptosis and necrosis in pancreatic cancer cell lines. After irradiation, PK-1 and Panc-1 cells were likely to undergo necrosis, whereas MIAPaCa-2 cells underwent apoptosis. When DiO-stained immature DCs were co-incubated with DiI-stained irradiated MIAPaCa-2, it was observed under fluorescent microscopy that DCs phagocytized dead tumor cells as early as 4 h after co-incubation. The DCs’ phagocytosis of irradiated tumor cells was also confirmed by flow cytometry. These results suggest that irradiated pancreatic cancer cells, which undergo both apoptosis and necrosis, could be a good source of tumor-associated antigens for cross-presentation by DCs.