Extremely low frequency (ELF) magnetic fields enhance chemically induced formation of apurinic/apyrimidinic (AP) sites in A172 cells
Extremely low frequency (ELF) magnetic fields enhance chemically induced formation of apurinic/apyrimidinic (AP) sites in A172 cells
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DOI：
10.1080/09553000701616064
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Miyakoshi, Junji
中科院分区：
文献类型：
--
作者：
Koyama, Shin;Sakurai, Tomonori;Miyakoshi, Junji
目的：为了检测极低频（ELF）磁场的作用，在暴露于ELF磁场之后测量人胶质瘤A172细胞中脱嘌呤/脱嘧啶（AP）位点的数目。细胞单独暴露于ELF磁场，暴露于遗传毒性剂（甲基甲烷磺酸盐（MMS）和过氧化氢（H2 O2）），或与遗传毒性剂一起暴露于ELF磁场。暴露后，提取DNA，AP sites的数量进行了measured.Results：有没有在AP网站的数量之间的细胞暴露于ELF磁场和假对照组之间的差异。单独使用MMS或H2 O2时，AP位点的数量随着治疗时间的延长而增加。暴露于ELF磁场与遗传毒性剂相结合增加AP-网站的水平相比，遗传毒性agents.Conclusions：我们的研究结果表明，由MMS或H2 O2诱导的AP网站的数量增加暴露于ELF磁场在5毫特斯拉（mT）。这可能发生，因为这样的暴露可以增强活性或延长自由基对的寿命。
Purpose: To detect the effects of extremely low frequency (ELF) magnetic fields, the number of apurinic/apyrimidinic (AP) sites in human glioma A172 cells was measured following exposure to ELF magnetic fields.Materials and methods: The cells were exposed to an ELF magnetic field alone, to genotoxic agents (methyl methane sulfonate (MMS) and hydrogen peroxide (H2O2)) alone, or to an ELF magnetic field with the genotoxic agents. After exposure, DNA was extracted, and the number of AP sites was measured.Results: There was no difference in the number of AP sites between cells exposed to an ELF magnetic field and sham controls. With MMS or H2O2 alone, the number of AP sites increased with longer treatment times. Exposure to an ELF magnetic field in combination with the genotoxic agents increased AP-site levels compared with the genotoxic agents alone.Conclusions: Our results suggest that the number of AP sites induced by MMS or H2O2 is enhanced by exposure to ELF magnetic fields at 5 millitesla (mT). This may occur because such exposure can enhance the activity or lengthen the lifetime of radical pairs.