Bioavailability of Ellagic Acid After Single Dose Administration Using HPLC
Bioavailability of Ellagic Acid After Single Dose Administration Using HPLC
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使用 HPLC 测定单剂量给药后鞣花酸的生物利用度
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
S. A. Oran
中科院分区：
文献类型：
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作者：
A. Hamad;W. Al;S. Janakat;S. A. Oran
摘要：鞣花酸是一种复杂的平面分子，具有多种抗癌活性。鞣花酸起到清道夫的作用，“结合”致癌化学物质，使其失去活性。它还具有抗细菌、抗病毒的特性。测定了20名健康受试者在0.15、0.3、1、1.30、2、3、6、8和12h内的血药浓度，给出了20名健康受试者给药40 mg后的血药浓度-时间曲线。采用反相Lichrospher 100 RP-18.5µm(250×4.6 mm i.d)色谱柱，甲醇为流动相，建立了测定低含量鞣花酸的等度反相液相色谱方法。用紫外检测器在254 nm波长处检测。标准EA的校正曲线与浓度在20-1000 ng/ml范围内线性关系良好，相关系数r=0.9997，血中鞣花酸(C)浓度最高。最大吸收峰浓度为200.15±26.66 ng/ml，平均血清消除半衰期为8.4h±1.4h，口服后50%以上的鞣花酸与血浆蛋白结合，主要在胃和肠道吸收。因此，一粒胶囊三次全天具有药物生物利用度。
3 Abstract: Ellagic Acid (EA) is a complex planar molecule which demonstrates a variety of anticarcinogenic activities. Ellagic Acid acts as a scavenger to "bind" cancer-causing chemicals, making them inactive. It also has anti-bacterial, anti-viral properties. The concentration of EA was assessed in serum of twenty healthy volunteers over time interval from 0.15, 0.3, 1, 1.30, 2, 3, 6, 8 and 12 h. The serum concentration-time profile is presented after administration of 40 mg of ellagic acid to 20 healthy volunteers. Isocratic reversed phase liquid chromatographic method was developed for determination of low quantity of Ellagic Acid (EA) on reversed phase Lichrospher 100 RP-18, 5 µm (250 x 4.6 mm i.d) column and using methanol as mobil e phase. The eluted EA was detected by UV set at 254 nm. Calibration curve for standard EA is linear with correlation coefficient of 0.9997 for detector response plotted against concentration range from 20-1000 ng/ml. The concentration of ellagic acid (C ) was highest in blood one hour (T ) after oral administration. max max It was 200.15±26.66 ng/ml., the mean serum elimination half-life was about 8.4±1.4 h. More than 50% of ellagic acid boud to plasma protein following oral administration, absorption of ionized ellagic acid occurs in stomach and intestine. Therefore, one capsule three times has drug bioavailability through all day.
影响因子：
5.8
作者：
Whitley, AC;Stoner, GD;Walle, T
通讯作者：
Walle, T