Negative Regulation of Autophagy by Sulfide Is Independent of Reactive Oxygen Species
Negative Regulation of Autophagy by Sulfide Is Independent of Reactive Oxygen Species
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DOI：
10.1104/pp.16.00110
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发表时间：
2016-06-01
期刊：
影响因子：
7.4
通讯作者：
Gotor, Cecilia
中科院分区：
文献类型：
--
作者：
Laureano-Marin, Ana M.;Moreno, Inmaculada;Gotor, Cecilia
在哺乳动物和最近的植物系统中积累的实验证据导致我们对硫化氢在生命过程中所起作用的理解发生了变化。在植物中，硫化氢缓解压力并调节重要的植物过程，如光合作用，气孔运动和自噬，尽管其潜在机制尚不清楚。在这项研究中，我们提供了新的实验证据，与我们以前的发现一起，证明了硫化氢在调节自噬中的作用。我们使用与自噬体相关的绿色荧光蛋白荧光和ATG 8蛋白的免疫印迹分析来显示硫化物（而没有其他分子，如含硫分子或铵）能够抑制氮剥夺下拟南芥（Arabidopsis thaliana）根中诱导的自噬。我们的研究结果表明，硫化物是无法消除活性氧物种产生的氮限制，在建立良好的还原剂。此外，还原剂不能抑制自噬体和ATG 8蛋白形式的积累到与硫化物相同的程度。因此，我们得出结论，硫化物抑制自噬通过一种机制，是独立的氧化还原条件。
Accumulating experimental evidence in mammalian, and recently plant, systems has led to a change in our understanding of the role played by hydrogen sulfide in life processes. In plants, hydrogen sulfide mitigates stress and regulates important plant processes such as photosynthesis, stomatal movement, and autophagy, although the underlying mechanism is not well known. In this study, we provide new experimental evidence that, together with our previous findings, demonstrates the role of hydrogen sulfide in regulating autophagy. We used green fluorescent protein fluorescence associated with autophagic bodies and immunoblot analysis of the ATG8 protein to show that sulfide (and no other molecules such as sulfur-containing molecules or ammonium) was able to inhibit the autophagy induced in Arabidopsis (Arabidopsis thaliana) roots under nitrogen deprivation. Our results showed that sulfide was unable to scavenge reactive oxygen species generated by nitrogen limitation, in contrast to well-established reducers. In addition, reducers were unable to inhibit the accumulation of autophagic bodies and ATG8 protein forms to the same extent as sulfide. Therefore, we conclude that sulfide represses autophagy via a mechanism that is independent of redox conditions.