Oncostatin M induces functional and structural impairment of blood-brain barriers comprised of rat brain capillary endothelial cells
Oncostatin M induces functional and structural impairment of blood-brain barriers comprised of rat brain capillary endothelial cells
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DOI：
10.1016/j.neulet.2008.06.030
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发表时间：
2008-08-22
影响因子：
2.5
通讯作者：
Kataoka, Yasufumi
中科院分区：
文献类型：
--
作者：
Takata, Fuyuko;Sumi, Noriko;Kataoka, Yasufumi
制瘤素 M (OSM) 是白细胞介素 6 家族的成员，由外周血中的单核细胞和巨噬细胞以及大脑中的小胶质细胞产生。本研究旨在阐明 OSM 在由大鼠脑毛细血管内皮细胞 (RBEC) 组成的血脑屏障 (BBB) 功能中的调节作用。 OSM 以浓度和时间依赖性方式降低 RBEC 单层的跨内皮电阻。在 OSM 处理的 RBEC 中，ZO-1 和claudin-5 的免疫细胞化学观察结果显示，沿着细胞之间的连接处呈拉链状和/或之字形，与载体处理的培养物中的平滑和线性形状形成鲜明对比。当用抗 OSM 抗体预处理 RBEC 时，OSM 未能引起这些变化。在大脑和外周血中产生 OSM 的细胞成分可能与 BBB 的功能和结构损伤有关。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
Oncostatin M (OSM), a member of the interleukin-6 family, is produced by monocytes and macrophages in the peripheral blood and microglia in the brain. The present study aimed to elucidate a regulatory role of OSM in the functions of blood-brain barrier (BBB) comprised of rat brain capillary endothelial cells (RBECs). OSM decreased the transendothelial electrical resistance of RBEC monolayers in a concentration-and time-dependent manner. Immunocytochemical observations of ZO-1 and claudin-5 in OSM-treated RBECs showed a zipper-like and/or zigzag shape along the junctions between cells, in contrast with the smooth and linear shape in vehicle-treated cultures. When RBECs were pre-treated with anti-OSM antibody, OSM failed to evoke these changes. The cellular constituents producing OSM in the brain and peripheral blood could be implicated in the functional and structural impairment of the BBB. (C) 2008 Elsevier Ireland Ltd. All rights reserved.