Effects of interleukin-1 beta, interleukin-6, and tumor necrosis factor on sensitivity of dorsal root ganglion and peripheral receptive fields in rats
Effects of interleukin-1 beta, interleukin-6, and tumor necrosis factor on sensitivity of dorsal root ganglion and peripheral receptive fields in rats
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DOI：
10.1007/s00586-005-0058-8
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发表时间：
2006-10-01
影响因子：
2.8
通讯作者：
Weinstein, James N.
中科院分区：
文献类型：
--
作者：
Oezaktay, A. Cueneyt;Kallakuri, Srinivasu;Weinstein, James N.
本研究旨在描述低剂量（0.5-5 ng）促炎细胞因子白细胞介素-1 β（IL-1 β）、白细胞介素-6（IL-6）和肿瘤坏死因子（TNF）对大鼠背根神经节（DRG）神经活动的影响。本研究的目的是研究细胞因子（IL-1 β，IL-6和TNF）对DRG体感神经反应的影响。由受损椎间盘释放的炎性细胞因子可能在神经末梢、轴突和神经细胞体的疼痛产生中起关键作用。突出的椎间盘组织已经显示出释放IL-1 β、IL-6、TNF和其他痛觉化学物质。它们对椎间盘和邻近组织中的神经末梢的作用可能导致腰痛，它们对背根轴突和神经节的作用可能导致坐骨神经痛。通过电生理技术研究暴露的腰椎DRG。将假手术（矿物油）、对照（载体溶液）或剂量为0.5、1和5 ng的IL-1 β、IL-6或TNF应用于DRG。基线放电率以及机械敏感性的DRG和外周感受野进行了评价超过30分钟。IL-1 β在1纳克的应用导致放电率的增加，5纳克导致机械敏感性的DRG在第二组单位增加。同样，在1 ng TNF应用后，第II组单位也显示DRG和外周感受野的机械敏感性增加。在低剂量下，观察到IL-1 β和TNF敏感的感受野。在第二组单位中观察到的反应表明，传入单位的亚群可能具有长期影响，改变中枢神经系统的感觉输入。这项研究为细胞因子在炎症后调节传入活动中的作用提供了额外的证据。
This study was designed to characterize the effects of low doses (0.5-5 ng) of pro-inflammatory cytokines, interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor (TNF), on the neural activity of dorsal root ganglion (DRG) in rats. The purpose of this study was to examine the effects of cytokines (IL-1 beta, IL-6, and TNF) on the somatosensory neural response of DRG. The release of inflammatory cytokines by an injured disc may play a critical role in pain production at nerve endings, axons, and nerve cell bodies. Herniated disc tissue has been shown to release IL-1 beta, IL-6, TNF, and other algesic chemicals. Their effects on nerve endings in disc and adjacent tissue may lead to low back pain and their effects on dorsal root axons and ganglia may lead to sciatica. Exposed lumbar DRGs were investigated by electrophysiologic techniques. Sham (mineral oil), control (carrier solution), or IL-1 beta, IL-6, or TNF at doses of 0.5, 1, and 5 ng were applied over the DRG. Baseline discharge rates as well as mechanosensitivity of the DRG and peripheral receptive fields were evaluated over 30 min. Applications of IL-1 beta at 1 ng resulted in an increase in the discharge rate, 5 ng resulted in an increased mechanosensitivity of the DRG in group II units. Similarly, after 1 ng TNF applications, group II units also showed an increase in mechanosensitivity of DRG and peripheral receptive fields. At low doses IL-1 beta and TNF sensitization of receptive fields were observed. The responses observed in the group II units indicate that a sub-population of afferent units might have long-term effects modifying the sensory input to the central nervous system. This study provides added evidence to the role of cytokines in modulating afferent activity following inflammation.