INTESTINAL-ABSORPTION OF BOTULINUM TOXINS OF DIFFERENT MOLECULAR SIZES IN RATS
INTESTINAL-ABSORPTION OF BOTULINUM TOXINS OF DIFFERENT MOLECULAR SIZES IN RATS
复制标题
DOI：
10.1128/iai.17.3.491-496.1977
复制
发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
SAKAGUCHI, G
中科院分区：
文献类型：
--
作者：
SUGII, S;OHISHI, I;SAKAGUCHI, G
将B型16S（L）毒素注射到大鼠结扎的十二指肠后10-12小时，在通过胸导管插管抽取的淋巴液中发现所施用的总毒性的0.01-0.1%。恢复率比给予 B 型 12S (M) 或 7S (S) 毒素的大鼠高 50-100 倍。在同一时期，无论施用的毒素的分子大小如何，都有 0.6-1.5% 的特异性抗原被回收。在 B-L 或 B-M 毒素受体的淋巴中，检测到了相当数量的有毒和无毒成分，表明未解离的祖毒素分子通过肠壁被吸收。虽然有毒成分失去了毒性，但淋巴中出现的B-M毒素的2种成分重新组装，重建了12S分子，而B-L毒素的2种成分却没有，但有毒成分仍然具有活性。 B-L、B-M 和 B-S 型毒素与体外暴露于大鼠淋巴（pH 8.2）时表现出相似的稳定性，但 B-L 毒素对肠液（pH 7.0）表现出比 B-M 毒素高得多的稳定性。因此，十二指肠内注射肉毒毒素的大鼠的淋巴毒性并不取决于吸收速率，而是取决于在肠道中的稳定性。
At 10-12 h after injection of type B 16S (L) toxin into the ligated duodenum of rats, 0.01-0.1% of the total toxicity administered was found in the lymph drawn by cannulation of the thoracic duct. The recovery was 50-100 times higher than that of the rat given type B 12S (M) or 7S (S) toxin. During the same period 0.6-1.5% of the specific antigens were recovered, regardless of the molecular size of the toxin that was administered. In lymph of the B-L or B-M toxin recipient, the toxic and nontoxic components were detected in comparable quantities, indicating that the undissociated progenitor toxin molecule is absorbed through the intestinal wall. Although the toxic component lost its toxic activity, the 2 components of B-M toxin appearing in lymph reassembled to reconstruct the 12S molecule, whereas those of B-L toxin did not, although the toxic component was still active. Type B-L, B-M and B-S toxins showed similar stabilities to in vitro exposure to rat lymph (pH 8.2), but B-L toxin showed a considerably higher stability to intestinal juice (pH 7.0) than did B-M toxin. Thus, the toxicity of lymph of rats administered botulinum toxin intraduodenally does not depend on the rate of absorption, but on the stability in the intestine.