Quail myoD is regulated by a complex array of cis-acting control sequences.
Quail myoD is regulated by a complex array of cis-acting control sequences.
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鹌鹑 myoD 受到一系列复杂的顺式作用控制序列的调节。
DOI：
10.1006/dbio.1995.1192
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
EmersonJr,CP
中科院分区：
文献类型：
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作者：
Pinney,DF;delaBrousse,FC;Faerman,A;Shani,M;Maruyama,K;EmersonJr,CP
Quail myoD(QmyoD)是在体节中表达的最早的myoD家族成员，其转录是对早期发育信号的响应。我们研究了QmyoD的转录调控，以确定组织特异性和正确的发育表达所需的顺式作用序列。对QmyoD基因座进行了分离和测序，并对其调控特性进行了研究。我们鉴定了顺式作用调控序列的三个不同区域，它们控制了报告基因构建体的表达，DNA导入细胞系和培养的原代细胞。第一个是基因上游11.5kb的一个复杂的远端控制区(DCR)，包含三个可分离的增强子活性。其中两种DCR增强子活性是组织特异性的，可以自动激活。此外，这两个相同的增强子和整个DCR在转基因小鼠中直接表达报告基因的体节和肌肉特异性。对DCR增强子的序列分析显示了E-box簇、MEF2结合基序和与人myoD增强子的序列同源性。其次，启动子区域有在肌肉和非肌肉细胞中直接表达的正向序列，以及在非肌肉细胞中特异抑制表达的序列。第三个控制区PR位于转录起始点的-3.3到-5kb之间，指导培养细胞中肌肉的特异性表达。这一分析表明，QmyoD具有多个控制区，并且MyoD调控的一些特征在哺乳动物和鸟类之间是保守的。
Quail myoD (QmyoD) is the earliest myoD family member expressed in quail somites and its transcription is initiated in response to early developmental signals. We have investigated the transcriptional regulation of QmyoD to define the cis-acting sequences required for tissue-specific and correct developmental expression. The QmyoD gene locus was isolated and sequenced and its regulatory properties were characterized. We identified three distinct regions of cis-acting regulatory sequences that control the expression of reporter gene constructs following DNA transfection into cell lines and cultured primary quail cells. The first, a complex distal control region (DCR), 11.5 kb upstream of the gene, contains three separable enhancer activities. Two of these DCR enhancer activities are tissue specific and can be autoactivated. In addition, these same two enhancers and the entire DCR direct somite- and muscle-specific expression of a reporter gene in transgenic mice. Sequence analysis of the DCR enhancers reveals clusters of E-boxes, MEF2 binding motifs, and stretches of sequence identity with the human myoD enhancer. Second, the promoter region has sequences which act positively to direct expression in both muscle and nonmuscle cells as well as sequences that repress expression specifically in nonmuscle cells. The third control region, the PR, is located -3.3 to -5 kb from the transcription start site and directs muscle-specific expression in cultured cells. This analysis demonstrates that QmyoD has multiple control regions and that some features of myoD regulation are conserved between mammals and birds.