Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells.
Overexpression of LINC00673 Promotes the Proliferation of Cervical Cancer Cells.
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LINC00673过表达促进宫颈癌细胞增殖
DOI：
10.3389/fonc.2021.669739
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发表时间：
2021
影响因子：
4.7
通讯作者：
Xu JP
中科院分区：
文献类型：
--
作者：
Huang SK;Ni RX;Wang WJ;Wang D;Zhao M;Lei CZ;Sun XJ;Huang CZ;Bai P;Che YQ;Xu JP
研究LINC00673在宫颈癌和宫颈上皮内瘤变(CIN)中的表达，探讨LINC00673在宫颈癌发生发展中的作用。采用RT-qPCR方法检测宫颈癌患者、宫颈上皮内瘤变患者和健康体检者血清中LINC00673的表达。通过体外和体内实验分析LINC00673在宫颈癌细胞中的作用。结果表明，宫颈癌患者血清LINC00673水平最高，CIN患者次之，健康对照组次之。体外实验表明，LINC00673过表达促进了HeLa和SiHA细胞的增殖和细胞周期进程。体内实验表明，皮下注射LINC00673过表达的HeLa细胞裸鼠的肿瘤重量和体积均大于注射对照细胞的裸鼠(P<；0.05)。Western blotting显示，在LINC00673过表达的HeLa和SiHA细胞中，细胞周期相关蛋白Cyclin A2和Cyclin E以及间质相关蛋白Snail和N-cadherin表达上调，而P53信号通路相关蛋白表达下调。LINC00673在宫颈癌的发生发展中起重要作用，可能成为宫颈癌治疗的新靶点。
To study the expression of LINC00673 in cervical cancer and cervical intraepithelial neoplasia (CIN) and to explore the role of LINC00673 in the development of cervical cancer. The expression of LINC00673 in serum from cervical cancer patients, CIN patients, and healthy participants was detected by RT-qPCR. The function of LINC00673 in cervical cancer cells was analyzed using in vitro and in vivo experiments. Our results revealed that serum LINC00673 levels were highest in cervical cancer patients, followed by patients with CIN and healthy controls. In vitro experiments demonstrated that overexpression of LINC00673 enhanced the proliferation and cell cycle progression of HeLa and SiHa cells. In vivo experiments showed that the tumor weight and volume of nude mice subcutaneously injected with LINC00673-overexpressing HeLa cells were larger than those of nude mice injected with control cells (P < 0.05). Western blotting showed that cell cycle-related proteins cyclin A2 and cyclin E and interstitial-associated proteins Snail and N-cadherin were upregulated and p53 signaling pathway-related proteins were downregulated in LINC00673-overexpressing HeLa and SiHa cells. LINC00673 plays an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.
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DOI：
10.1016/s1470-2045(20)30674-4
发表时间：
2021-03
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Ryzhov A;Corbex M;Piñeros M;Barchuk A;Andreasyan D;Djanklich S;Ghervas V;Gretsova O;Kaidarova D;Kazanjan K;Mardanli F;Michailovich Y;Ten E;Yaumenenka A;Bray F;Znaor A
通讯作者：
Znaor A
影响因子：
64.5
作者：
van Werven FJ;Neuert G;Hendrick N;Lardenois A;Buratowski S;van Oudenaarden A;Primig M;Amon A
通讯作者：
Amon A
影响因子：
3.3
作者：
Vitkauskaite, Agne;Urboniene, Daiva;Vaitkiene, Daiva
通讯作者：
Vaitkiene, Daiva
影响因子：
64.5
作者：
Rinn, John L.;Kertesz, Michael;Chang, Howard Y.
通讯作者：
Chang, Howard Y.
LINC00673被YY1激活并通过miR-515-5p/MARK4/Hippo信号通路促进乳腺癌细胞增殖
DOI：
10.1186/s13046-019-1421-7
发表时间：
2019-10-17
影响因子：
11.3
作者：
Qiao, Kun;Ning, Shipeng;Pang, Da
通讯作者：
Pang, Da