Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis
Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis
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DOI：
10.1109/transducers.2015.7181234
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
M. Tabata;H. Yang;F. Mannan;Y. Katayama;T. Goda;A. Matsumoto;A. Seichi;K. Suzuki;Y. Miyahara
中科院分区：
文献类型：
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作者：
M. Tabata;H. Yang;F. Mannan;Y. Katayama;T. Goda;A. Matsumoto;A. Seichi;K. Suzuki;Y. Miyahara
无标记的微型电化学装置已经被开发用于监测核酸扩增。我们成功地监测释放的质子在扩增反应中，以实时的方式使用微型pH传感器，并证明了核酸的定量检测。由于离子敏感场效应晶体管（ISFET）（-58.0 mV/pH）和铱/氧化铱（Ir/IrOx）电极作为pH传感器的灵敏度（-57.4 mV/pH）显示接近理论值（-59.2 mV/pH），等温核酸扩增和电化学检测之间的组合与潜在稳定的测量兼容。所提出的系统将是具有成本效益的和便携式的，并可能在临床应用中实现，如诊断传染病和癌症。
Label-free miniaturized electrochemical devices have been developed to monitor the nucleic acid amplification. We successfully monitored released protons during amplification reaction in real-time manner using miniaturized pH sensors and demonstrated quantitative detection of nucleic acid. Because the sensitivity of ion-sensitive field effect transistor (ISFET) (-58.0 mV/pH) and iridium/ iridium oxide (Ir/IrOx) electrode (-57.4 mV/pH) as a pH sensor showed close values to theoretical value (-59.2 mV/pH), the combination between isothermal nucleic acid amplification and the electrochemical detection was compatible with potentially stable measurement. The proposed system would be cost-effective and portable, and potentially implemented in clinical use such as diagnosis of infectious disease and cancer.