Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope
Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope
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DOI：
10.1364/oe.16.007224
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发表时间：
2008-05-12
期刊：
影响因子：
3.8
通讯作者：
Solgaard, Olav
中科院分区：
文献类型：
--
作者：
Ra, Hyejun;Piyawattanametha, Wibool;Solgaard, Olav
我们提出了一个手持双轴共焦显微镜，是基于一个二维微机电系统（MEMS）扫描仪。它在488 nm波长下进行反射和荧光成像，具有三维成像能力。完全封装的显微镜直径为10 mm，以4 Hz的帧率采集图像，最大视场为400 μ m x 260 μ m。手持探头的横向和轴向分辨率分别为1.7 μ m和5.8 μ m。在转基因小鼠体内证明了执行真实的时间小动物成像的能力。(C)2008年美国光学学会。
We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 mu m x 260 mu m. The transverse and axial resolutions of the handheld probe are 1.7 mu m and 5.8 mu m, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice. (C) 2008 Optical Society of America.