Caloric restriction augments ROS defense in S-cerevisiae, by a Sir2p independent mechanism
Caloric restriction augments ROS defense in S-cerevisiae, by a Sir2p independent mechanism
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DOI：
10.1080/10715760400022343
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发表时间：
2005-01-01
影响因子：
3.3
通讯作者：
Roy, N
中科院分区：
文献类型：
--
作者：
Agarwal, S;Sharma, S;Roy, N
衰老与活性氧（ROS）的产生增加和氧化诱导的细胞内结构和膜损伤有关。热量限制（CR）已被证明可以延缓各种物种的衰老。尽管CR的机制仍有待明确阐明，但在几种模型系统中，氧化损伤的减少已显示出延长寿命。相反，一般认为，ROS生产减少CR，这篇文章提供的证据表明，不仅耗氧量，但ROS生产的热量限制条件下增强。为了解CR抗衰老作用的生物学机制，研究了清除酶的作用。结果表明，CR中超氧化物歧化酶（SOD 1、SOD 2）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）均过表达。我们进一步研究了Sir2在清除酶激活中的作用，Sir2是CR反应的潜在效应子。没有显着差异，发现在CR介导的SOD和过氧化氢酶的激活Sir2的情况下。我们的研究结果表明，在CR清除酶被激活的Sir2独立的方式。
Aging is associated with increased production of reactive oxygen species (ROS) and oxidation-induced damage to intracellular structures and membranes. Caloric restriction (CR) has been demonstrated to delay aging in a variety of species. Although the mechanisms of CR remain to be clearly elucidated, reductions in oxidative damage have been shown to increase lifespan in several model systems. Contrary to the general belief that ROS production is reduced in CR, this article provides evidence that not only oxygen consumption but ROS production is enhanced in the caloric restricted condition. To understand the biological mechanism underlying the anti aging action of CR, the role of scavenging enzymes was studied. It was found that super oxide dismutase (SOD 1 and SOD2), catalase and glutathione peroxidase (GPx) all are over expressed in CR. We further investigated the role of Sir2, a potential effector of CR response in the activation of scavenging enzymes. No marked difference was found in CR mediated activation of SOD and catalase in the absence of Sir2. Our results suggest that in CR scavenging enzymes are activated by a Sir2 independent manner.