Development and validation of a ultra performance LC-ESI/MS method for analysis of metabolic phenotypes of healthy men in day and night urine samples.
Development and validation of a ultra performance LC-ESI/MS method for analysis of metabolic phenotypes of healthy men in day and night urine samples.
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DOI：
10.1002/jssc.200800326
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发表时间：
2008-09
影响因子：
3.1
通讯作者：
Xi-jun Wang;Haitao Lv;Guangmei Zhang;Wen-jun Sun;Dixin Zhou;Guozheng Jiao;Yang Yu
中科院分区：
文献类型：
--
作者：
Xi-jun Wang;Haitao Lv;Guangmei Zhang;Wen-jun Sun;Dixin Zhou;Guozheng Jiao;Yang Yu
建立并验证了一种用于分析健康男性尿液代谢物的超高效液相色谱-四极TOF/MS联用技术(UPLC-QTOF/MS)。使用主成分分析(PCA)的数据分析显示，健康男性白天和晚上的尿液代谢表型存在差异。健康男性夜间尿液中质量电荷比(m/z)分别为310.24(5.35min)、286.24(4.74min)和310.24(5.63min)的阳离子较白天升高。健康男性日间尿液中负离子浓度升高分别为m/z 167.02(0.66min)、263.12(2.55min)和191.03(0.73min)，而m/z 212.01(4.77min)则低于夜间。离子m/z 212.01(4.77min)、191.03(0.73min)和310.24(5.35min)初步对应于吲哚硫酸盐、柠檬酸和N-乙酰神经氨酸，进一步支持了健康男性昼夜尿液表型差异的存在，这可能与健康男性肠道微生物区系活动、三羧酸循环速度和唾液酸生物合成活性显著不同有关，受哺乳动物生物钟昼夜节律的调节。
Ultra-performance LC coupled to quadrupole TOF/MS (UPLC-QTOF/MS) in positive and negative ESI was developed and validated to analyze metabolite profiles for urine from healthy men during the day and at night. Data analysis using principal components analysis (PCA) revealed differences between metabolic phenotypes of urine in healthy men during the day and at night. Positive ions with mass-to-charge ratio (m/z) 310.24 (5.35 min), 286.24 (4.74 min) and 310.24 (5.63 min) were elevated in the urine from healthy men at night compared to that during the day. Negative ions elevated in day urine samples of healthy men included m/z 167.02 (0.66 min), 263.12 (2.55 min) and 191.03 (0.73 min), whilst ions m/z 212.01 (4.77 min) were at a lower concentration in urine of healthy men during the day compared to that at night. The ions m/z 212.01 (4.77 min), 191.03 (0.73 min) and 310.24 (5.35 min) preliminarily correspond to indoxyl sulfate, citric acid and N-acetylneuraminic acid, providing further support for an involvement of phenotypic difference in urine of healthy men in day and night samples, which may be associated with notably different activities of gut microbiota, velocity of tricarboxylic acid cycle and activity of sialic acid biosynthesis in healthy men as regulated by circadian rhythm of the mammalian bioclock.