The course of Mycobacterium tuberculosis infection in the lungs of mice lacking expression of either perforin- or granzyme-mediated cytolytic mechanisms
The course of Mycobacterium tuberculosis infection in the lungs of mice lacking expression of either perforin- or granzyme-mediated cytolytic mechanisms
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DOI：
10.1128/iai.65.4.1317-1320.1997
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发表时间：
1997-04-01
影响因子：
3.1
通讯作者：
Orme, IM
中科院分区：
文献类型：
--
作者：
Cooper, AM;DSouza, C;Orme, IM
CD8T细胞已被证明对小鼠的结核分枝杆菌感染具有保护作用。这些细胞已被证明对结核分枝杆菌感染的细胞具有细胞溶解作用，并且还被证明能释放保护性细胞因子伽玛干扰素来响应分枝杆菌抗原。因此，目前还不清楚这些细胞是如何调节其保护性反应的。为了剖析这个问题，我们比较了对照组、穿孔素基因敲除小鼠和颗粒酶基因敲除小鼠暴露在现实的肺部途径中的结核分枝杆菌感染过程。不能表达这两种分子中的任何一种限制了主要裂解途径的表达，但似乎不会影响感染过程或导致任何明显的组织学差异。这些数据似乎排除了CD8 T细胞在肺中的溶解作用，因此倾向于表明另一种类型的机制，如这些细胞分泌的细胞因子，是它们的主要作用模式。
CD8 T cells have been shown to be protective against Mycobacterium tuberculosis infections in the mouse. These cells have been shown to be cytolytic toward M. tuberculosis-infected cells and have also been shown to release the protective cytokine gamma interferon in response to mycobacterial antigen. It has therefore been unclear how these cells mediate their protective response. To dissect this problem, we compared the courses of M. tuberculosis infections in control, perforin gene-knockout, and granzyme gene-knockout mice exposed by the realistic pulmonary route. The inability to express either of these molecules limits the expression of the major lytic pathway but does not appear to influence the course of the infection or result in any discernible histologic differences. These data seem to rule against a lytic role for CD8 T cells in the lungs and hence tend to suggest instead that another type of mechanism, such as cytokine secretion by these cells, is their primary mode of action.