Green and low-cost synthesis of nitrogen-doped graphene-like mesoporous nanosheets from the biomass waste of okara for the amperometric detection of vitamin C in real samples
Green and low-cost synthesis of nitrogen-doped graphene-like mesoporous nanosheets from the biomass waste of okara for the amperometric detection of vitamin C in real samples
复制标题
从豆渣生物质废物中绿色、低成本合成氮掺杂石墨烯类介孔纳米片，用于实际样品中维生素 C 的安培检测
DOI：
10.1016/j.talanta.2019.03.071
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Zhou Ming
中科院分区：
文献类型：
--
作者：
Sha Tianze;Liu Jingju;Sun Mimi;Li Lei;Bai Jing;Hu Zongqian;Zhou Ming
本工作首次以奥卡拉生物质废料为原料合成了低成本的氮掺杂类石墨烯介孔纳米片（N-GMNs），用于构建非酶电流型维生素C生物传感器。与传统玻碳电极和碳纳米管修饰玻碳电极相比，N-GMNs修饰玻碳电极（N-GMNs/GCE）对维生素C的电氧化过电位明显降低，表明N-GMNs/GCE有望用于灵敏、选择性的维生素C非酶安培生物传感。作为维生素C的非酶电流型生物传感器，N-GMNs/GCE比GCE、CNTs/GCE或最近报道的一些基于纳米材料的电化学维生素C生物传感器显示出更高的灵敏度（144.65 μA mM−1cm−2）、更宽的线性范围（10-5640 μmol L − 1）和更低的检测限（0.51 μmol L−1）。特别是对市售饮料、维生素C注射液和市售果汁等真实的样品中维生素C的含量进行了测定，结果令人满意。因此，以奥卡拉为原料制备纳米碳纳米管是一种绿色的方法，可以制备出先进、低成本的电极材料，用于维生素C检测的非酶电化学生物传感器。
In this work, the low-cost nitrogen-doped graphene-like mesoporous nanosheets (N-GMNs) was synthesized from the biomass waste of okara for the first time for the construction of a nonenzymatic amperometric vitamin C biosensor. The N-GMNs modified glassy carbon electrode (N-GMNs/GCE) shows much lower overpotential for the electrooxidation of vitamin C comparing to the traditional GCE as well as the GCE modified by carbon nanotubes (CNTs/GCE), indicating the promising of N-GMNs/GCE for the sensitive and selective nonenzymatic amperometric vitamin C biosensing. As a nonenzymatic amperometric biosensor for vitamin C, the N-GMNs/GCE shows a higher sensitivity (144.65 μA mM−1cm−2), a wider linear range (10–5640 μmol L−1) and a lower detection limit (0.51 μmol L−1) than GCE, CNTs/GCE or some of recently reported nanomaterials-based electrochemical vitamin C biosensors. Especially, the vitamin C concentration in real samples of commercial beverage, vitamin C injection and commercial juice can be determined by the proposed N-GMNs/GCE with satisfied results. Therefore, the utilization of okara as the raw material for the synthesis of nanostructured carbon of N-GMNs is a green method to fabricate an advanced and low-cost electrode material for developing the nonenzymatic electrochemical biosensor for vitamin C detection.